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Table 2 Effect of YE or MeJA concentration on cell growth parameters and costunolide accumulation

From: Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures

Treatments Fresh weight (mg) Dry weight (mg) Costunolide (ppm)
YE (mg/L)
 0 748.0 ± 1.5a(A) 78.0 ± 0.6a(A) 1.70 ± 0.15d(E)
 100 536.0 ± 5.0c(D) 43.0 ± 1.5bc(DE) 1.73 ± 0.01d(E)
 150 364.0 ± 3.1d(F) 39.0 ± 1.7c(EF) 2.56 ± 0.01c(D)
 200 730.7 ± 5.2a(B) 47.0 ± 3.1b(CD) 3.47 ± 0.01a(B)
 250 670.7 ± 10.3b(C) 49.0 ± 3.5b(C) 3.02 ± 0.01b(C)
p values (one-way ANOVA) [YE concentration] 0.000*** 0.000*** 0.000***
MeJA (mg/L)
 0 748.0 ± 0.6a(A) 78.0 ± 0.6a(A) 1.70 ± 0.2d(E)
 100 420.0 ± 1.5b(E) 58.0 ± 0.6b(B) 3.30 ± 0.6b(B)
 150 301.7 ± 0.9c(G) 36.0 ± 0.0c(F) 5.50 ± 0.6a(A)
 200 220.0 ± 3.6d(H) 28.0 ± 0.6d(G) 2.60 ± 0.2c(D)
 250 130.0 ± 0.0e(I) 4.2 ± 0.2e(H) 1.60 ± 0.6d(E)
p values (one-way ANOVA) [MeJA concentration] 0.000*** 0.000*** 0.000***
Two-way ANOVA/general linear model
 Elicitor type (A) 0.000*** 0.000*** 0.000***
 Elicitor concentration (B) 0.000*** 0.000*** 0.000***
 (A)*(B) 0.000*** 0.000*** 0.000***
  1. Means (n = 30) ± standard error (S.E.) with the same letter in a column are not statistically significant different from each other according to the Duncan’s multiple range test at p ≤ 0.05. ***p ≤ 0.001. Superscript small letters—differences between five concentrations for each elicitor type (either YE or MeJA) separately (one-way ANOVA). Superscript capital letters in parenthesis—differences between samples from the combined effect of two elicitor types and five elicitor concentrations (two-way ANOVA)