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Table 3 Gene Ontology (GO) and KEGG pathways classification of specific salinity–alkalinity up-regulated genes

From: Transcriptomic and proteomic analyses provide insights into the adaptive responses to the combined impact of salinity and alkalinity in Gymnocypris przewalskii

A. Biological process

GO ID

Description

Saline–alkaline up-regulated deps (18)

All (4696)

p-value

p-adjust

GO:0,048,771

Tissue remodeling

3 (16.67%)

19 (0.4%)

0.000044

0.019294

GO:0,051,384

Response to glucocorticoid

3 (16.67%)

23 (0.49%)

0.000080

0.019294

GO:0,034,614

Cellular response to reactive oxygen species

3 (16.67%)

25 (0.53%)

0.000103

0.019294

GO:0,006,816

Calcium ion transport

4 (22.22%)

85 (1.81%)

0.000252

0.019294

GO:0,010,519

Negative regulation of phospholipase activity

2 (11.11%)

8 (0.17%)

0.000383

0.019294

GO:0,032,717

Negative regulation of interleukin-8 production

2 (11.11%)

8 (0.17%)

0.000383

0.019294

GO:0,060,192

Negative regulation of lipase activity

2 (11.11%)

8 (0.17%)

0.000383

0.019294

GO:0,070,838

Divalent metal ion transport

4 (22.22%)

99 (2.11%)

0.000453

0.019294

GO:0,072,511

Divalent inorganic cation transport

4 (22.22%)

99 (2.11%)

0.000453

0.019294

GO:0,034,599

Cellular response to oxidative stress

3 (16.67%)

41 (0.87%)

0.000460

0.019294

GO:0,001,516

Prostaglandin biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:0,031,392

Regulation of prostaglandin biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:0,042,304

Regulation of fatty acid biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:0,046,456

Icosanoid biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:0,046,457

Prostanoid biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:1,901,570

Fatty acid derivative biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:2,001,279

Regulation of unsaturated fatty acid biosynthetic process

2 (11.11%)

9 (0.19%)

0.000492

0.019294

GO:1,901,701

Cellular response to oxygen-containing compound

3 (16.67%)

48 (1.02%)

0.000734

0.027216

GO:0,032,637

Interleukin-8 production

2 (11.11%)

12 (0.26%)

0.000895

0.029862

GO:0,032,677

Regulation of interleukin-8 production

2 (11.11%)

12 (0.26%)

0.000895

0.029862

GO:1,901,989

Positive regulation of cell cycle phase transition

2 (11.11%)

13 (0.28%)

0.001056

0.032011

GO:1,901,992

Positive regulation of mitotic cell cycle phase transition

2 (11.11%)

13 (0.28%)

0.001056

0.032011

GO:0,031,960

Response to corticosteroid

3 (16.67%)

57 (1.21%)

0.001216

0.034156

GO:0,032,309

Icosanoid secretion

2 (11.11%)

14 (0.3%)

0.001229

0.034156

GO:0,071,715

Icosanoid transport

2 (11.11%)

15 (0.32%)

0.001415

0.036297

GO:1,901,571

Fatty acid derivative transport

2 (11.11%)

15 (0.32%)

0.001415

0.036297

GO:0,006,979

Response to oxidative stress

4 (22.22%)

136 (2.9%)

0.001501

0.037092

GO:0,006,952

Defense response

7 (38.89%)

495 (10.54%)

0.001562

0.037216

GO:0,090,068

Positive regulation of cell cycle process

2 (11.11%)

17 (0.36%)

0.001824

0.041958

GO:0,001,776

Leukocyte homeostasis

2 (11.11%)

18 (0.38%)

0.002048

0.043514

GO:0,046,717

Acid secretion

2 (11.11%)

18 (0.38%)

0.002048

0.043514

GO:0,046,942

Carboxylic acid transport

3 (16.67%)

69 (1.47%)

0.002115

0.043514

GO:0,015,849

Organic acid transport

3 (16.67%)

70 (1.49%)

0.002205

0.043514

GO:0,006,636

Unsaturated fatty acid biosynthetic process

2 (11.11%)

19 (0.4%)

0.002283

0.043514

GO:0,045,931

Positive regulation of mitotic cell cycle

2 (11.11%)

19 (0.4%)

0.002283

0.043514

GO:0,006,693

Prostaglandin metabolic process

2 (11.11%)

20 (0.43%)

0.002531

0.045632

GO:0,006,956

Complement activation

2 (11.11%)

20 (0.43%)

0.002531

0.045632

GO:0,044,092

Negative regulation of molecular function

4 (22.22%)

159 (3.39%)

0.002671

0.046881

B. Molecular function

GO ID

Description

Saline–alkaline up-regulated deps (18)

All (4619)

p-value

p-adjust

GO:0,004,859

Phospholipase inhibitor activity

2 (11.11%)

9 (0.19%)

0.000508

0.043946

GO:0,008,094

DNA-dependent ATPase activity

2 (11.11%)

12 (0.26%)

0.000925

0.043946

GO:0,055,102

Lipase inhibitor activity

2 (11.11%)

15 (0.32%)

0.001462

0.046287

C. Cellular component

GO ID

Description

Saline–alkaline up-regulated deps (19)

All (4741)

p-value

p-adjust

GO:0,005,576

Extracellular region

8 (42.11%)

445 (9.39%)

0.000168

0.012632

GO:0,044,421

Extracellular region part

7 (36.84%)

427 (9.01%)

0.000884

0.033153

D. KEGG pathway enrichment

Pathway

Candidate genes with pathway annotation (11)

All genes with pathway annotation (3593)

P-value

Q -value

Pathway ID

Neuroactive ligand–receptor interaction

2 (18.18%)

13 (0.36%)

0.000653

0.050910

ko04080

Complement and coagulation cascades

2 (18.18%)

43 (1.2%)

0.007186

0.247888

ko04610

Herpes simplex infection

3 (27.27%)

152 (4.23%)

0.009534

0.247888

ko05168

Arginine biosynthesis

1 (9.09%)

16 (0.45%)

0.047973

0.307125

ko00220