Fig. 4.
From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
1-mL column validation runs of Capto Butyl ImpRes at the optimal condition of pH 4.0 for FabscFv-KiH (a) as well as Fab2scFv-KiH post-Protein A eluate with pH 4.3 wash (b) and pH 4.1 wash (c). The cumulative HMW (blue markers), LMW (grey markers) and monomeric (orange markers) species in the flow-through are represented as a function of the amount of bsAb loaded, with the initial percentage of each species represented in their respective colours in dotted lines