Table 1 Representative purity profile of cell culture supernatant (CCS) and post-Protein A eluates of FabscFv-KiH and Fab2scFv-KiH
From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
| Â | Â | Load | Monomer concentration (mg/mL) | HCP (ppm) | Purity (%) | ||
|---|---|---|---|---|---|---|---|
HMW | Mono | LMW | |||||
FabscFv-KiH | CCS | – | 0.69 | 1,357,822 | 30.8 | 35.5 | 33.8 |
Post-Protein A eluate | 31.5Â mg/mL-R | 5.00 | 1779 | 5.7 | 93.9 | 0.4 | |
Fab2scFv-KiH | CCS | – | 0.72 | 1,329,885 | 33.5 | 31.1 | 35.4 |
Post-Protein A eluate pH 4.3 wash | 30.5Â mg/mL-R | 2.74 | 1551 | 6.1 | 92.1 | 1.8 | |
Post-Protein A eluate pH 4.1 wash | 2.92 | 1526 | 6.5 | 92.1 | 1.4 | ||