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Table 1 Representative purity profile of cell culture supernatant (CCS) and post-Protein A eluates of FabscFv-KiH and Fab2scFv-KiH

From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

   Load Monomer concentration (mg/mL) HCP (ppm) Purity (%)
HMW Mono LMW
FabscFv-KiH CCS 0.69 1,357,822 30.8 35.5 33.8
Post-Protein A eluate 31.5 mg/mL-R 5.00 1779 5.7 93.9 0.4
Fab2scFv-KiH CCS 0.72 1,329,885 33.5 31.1 35.4
Post-Protein A eluate
pH 4.3 wash
30.5 mg/mL-R 2.74 1551 6.1 92.1 1.8
Post-Protein A eluate
pH 4.1 wash
2.92 1526 6.5 92.1 1.4