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Table 2 96-well plate screening conditions of Capto Butyl ImpRes, Capto adhere ImpRes and Capto S ImpAct for both FabscFv-KiH and Fab2scFv-KiH

From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

  Buffer pH Salt
Capto Butyl ImpRes Sodium citrate 4.0, 5.25, 6.5 20, 100, 200, 300, 400 mM Na-citrate
Capto adhere ImpRes 20 mM sodium citrate + 20 mM sodium phosphate 5.0, 5.75, 6.5 0, 50, 100, 150, 200, 250 mM NaCl
Capto S ImpAct 20 mM sodium citrate (pH 5.0–6.0)
20 mM sodium phosphate (pH 6.5–7.5)
5.0, 5.5, 6.0, 6.5, 7.0, 7.5 0, 50, 100 mM NaCl