Table 3 Analysis of the monomer recovery and purity profile of post-Capto S ImpAct eluate in comparison to the load of post-Capto Butyl ImpRes flow-through reflects on-column aggregation of FabscFv-KiH on Capto S ImpAct
From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
FabscFv-KiH | Monomer concentration (mg/mL) | Monomer recovery (%) | Purity (%) | |||
|---|---|---|---|---|---|---|
HMW | Mono | LMW | ||||
Load (Post-Capto Butyl ImpRes flow-through) | 1.76 | – | 2.7 | 96.3 | 1.0 | |
Post-Capto S ImpAct: pH 5.0, 0Â mM NaCl | Early fractions | 0.22 | 4.5 | 1.5 | 62.2 | 36.3 |
Mid fractions | 3.56 | 61.3 | 5.9 | 93.7 | 0.3 | |
Late fractions | 0.74 | 23.1 | 9.5 | 90.0 | 0.6 | |
Load (Post-Capto Butyl ImpRes flow-through) | 1.68 | – | 2.9 | 95.9 | 1.2 | |
Post-Capto S ImpAct: pH 5.0, 50Â mM NaCl | Early fractions | 0.32 | 6.6 | 1.5 | 66.6 | 31.9 |
Mid fractions | 3.35 | 57.7 | 4.6 | 95.0 | 0.4 | |
Late fractions | 0.59 | 20.3 | 13.8 | 85.7 | 0.5 | |
Load (Post-Capto Butyl ImpRes flow-through) | 1.68 | – | 2.9 | 95.6 | 1.5 | |
Post-Capto S ImpAct: pH 5.5, 50Â mM NaCl | Early fractions | 0.4 | 1.4 | 1.6 | 89.3 | 9.1 |
Mid fractions | 3.33 | 67.0 | 3.6 | 95.8 | 0.6 | |
Late fractions | 0.31 | 7.5 | 47.5 | 51.2 | 1.4 | |