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Table 3 Analysis of the monomer recovery and purity profile of post-Capto S ImpAct eluate in comparison to the load of post-Capto Butyl ImpRes flow-through reflects on-column aggregation of FabscFv-KiH on Capto S ImpAct

From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

FabscFv-KiH Monomer concentration (mg/mL) Monomer recovery (%) Purity (%)
HMW Mono LMW
Load (Post-Capto Butyl ImpRes flow-through) 1.76 2.7 96.3 1.0
Post-Capto S ImpAct:
pH 5.0, 0 mM NaCl
Early fractions 0.22 4.5 1.5 62.2 36.3
Mid fractions 3.56 61.3 5.9 93.7 0.3
Late fractions 0.74 23.1 9.5 90.0 0.6
Load (Post-Capto Butyl ImpRes flow-through) 1.68 2.9 95.9 1.2
Post-Capto S ImpAct:
pH 5.0, 50 mM NaCl
Early fractions 0.32 6.6 1.5 66.6 31.9
Mid fractions 3.35 57.7 4.6 95.0 0.4
Late fractions 0.59 20.3 13.8 85.7 0.5
Load (Post-Capto Butyl ImpRes flow-through) 1.68 2.9 95.6 1.5
Post-Capto S ImpAct:
pH 5.5, 50 mM NaCl
Early fractions 0.4 1.4 1.6 89.3 9.1
Mid fractions 3.33 67.0 3.6 95.8 0.6
Late fractions 0.31 7.5 47.5 51.2 1.4