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Table 5 Comparison between the effect of pH 6.5 and pH 6.8 on Capto adhere flow-through mode using FabscFv-KiH

From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

  FabscFv-KiH Load Monomer concentration (mg/mL) Step monomer recovery (%) Overall monomer recovery (%) HCP (ppm) Purity (%)
HMW Mono LMW
  CCS - 0.69 - - 1,357,822 30.8 35.5 33.8
Process 1 Post-Protein A eluate 31.5 mg/mL-R 5.00 91.1 91.1 1779 5.7 93.9 0.4
Polishing FT mode:
Capto Butyl ImpRes,
pH 4.0
40 mg/mL-R 1.26 88.2 80.4 180 1.7 98.1 0.2
Polishing FT mode:
Capto adhere,
pH 6.5
30 mg/mL-R 0.50 83.3 66.9 135 0.5 99.3 0.2
Process 2 Post-Protein A eluate 31.5 mg/mL-R 5.00 91.1 91.1 1779 5.7 93.9 0.4
Polishing FT mode:
Capto Butyl ImpRes,
pH 4.0
40 mg/mL-R 1.26 88.2 80.4 180 1.7 98.1 0.2
Polishing FT mode:
Capto adhere,
pH 6.8
30 mg/mL-R 0.45 75.0 60.3 81 0.4 99.4 0.2