Table 5 Comparison between the effect of pH 6.5 and pH 6.8 on Capto adhere flow-through mode using FabscFv-KiH
From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
| Â | FabscFv-KiH | Load | Monomer concentration (mg/mL) | Step monomer recovery (%) | Overall monomer recovery (%) | HCP (ppm) | Purity (%) | ||
|---|---|---|---|---|---|---|---|---|---|
HMW | Mono | LMW | |||||||
| Â | CCS | - | 0.69 | - | - | 1,357,822 | 30.8 | 35.5 | 33.8 |
Process 1 | Post-Protein A eluate | 31.5Â mg/mL-R | 5.00 | 91.1 | 91.1 | 1779 | 5.7 | 93.9 | 0.4 |
Polishing FT mode: Capto Butyl ImpRes, pH 4.0 | 40Â mg/mL-R | 1.26 | 88.2 | 80.4 | 180 | 1.7 | 98.1 | 0.2 | |
Polishing FT mode: Capto adhere, pH 6.5 | 30Â mg/mL-R | 0.50 | 83.3 | 66.9 | 135 | 0.5 | 99.3 | 0.2 | |
Process 2 | Post-Protein A eluate | 31.5Â mg/mL-R | 5.00 | 91.1 | 91.1 | 1779 | 5.7 | 93.9 | 0.4 |
Polishing FT mode: Capto Butyl ImpRes, pH 4.0 | 40Â mg/mL-R | 1.26 | 88.2 | 80.4 | 180 | 1.7 | 98.1 | 0.2 | |
Polishing FT mode: Capto adhere, pH 6.8 | 30Â mg/mL-R | 0.45 | 75.0 | 60.3 | 81 | 0.4 | 99.4 | 0.2 | |