Table 1 Summary of enzymes characteristics
From: Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Enzyme | CAZy family | Microorganism | Substrate | T optima (℃) | pH optima |
|---|---|---|---|---|---|
endo-1,4-β-Xylanase (EC 3.2.1.8) | GH11 | Neocallimastix patriciarum | Endo-hydrolysis of (1,4)-β-d-xylosidic linkages in xylans | 50 | 6.0 |
GH10 | Cellvibrio japonicus | 60 | 5.0 | ||
α-l-Arabinofuranosidase (EC 3.2.1.55) | GH43 | Bifidobacterium adolescentis | Highly specific hydrolysis of α-1,3-linked l-arabinofuranose residues from doubly substituted d-xylosyl or l-arabinosyl residues of arabinoxylans and branched arabinans, respectively | 50 | 6.0 |
GH51 | Aspergillus niger | Hydrolysis of α-1,2- and α-1,3-linked l-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate | 40 | 4.0 | |
exo-1,4-β-d-Xylosidase (EC 3.2.1.37) | GH43 | Selenomonas ruminantium | Hydrolysis of (1,4)-β-d-xylans and xylo-oligosaccharides to remove successive d-xylose residues from non-reducing termini | 50 | 5.0 |
α-d-Glucuronidase (EC 3.2.1.139) | GH67 | Geobacillus stearothermophilus | Hydrolysis of the α-1,2 glycosidic bond between d-glucuronic acid or its ether 4-O-methyl-d-glucuronic acid from the terminal non-reducing d-xylose residues of xylo-oligosaccharides (aldo-uronic acids) and xylan | 70 | 7.0 |