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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: A novel sustainable platform for scaled manufacturing of double-stranded RNA biopesticides

Fig. 2

Purification of AREV dsRNA. Method DPM1 was used to extract 700 bp (AREV4) dsRNAs from 1 mL culture induced for 18 h in the media A–E and extracted RNA from the media analysed by agarose gel electrophoresis: A lanes a, b, c, d and e: RNA sample from media A, media B, media C, media D and media E, respectively. For lanes f, g and h, media B purified RNA samples were treated as follows before agarose gel electrophoresis: f) added 1 µL RNase T1 and incubated for 10 min g) mixed with DMSO (50% final concentration), incubated at 90 °C for 1 min, added 1 µL RNase T1 and incubated for 10 min h) mixed with 50% DMSO and incubated at 90 °C for 1 min)

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