Fig. 3

Optimisation of purification method to reduce background multimeric dsRNA (DPM2). Method DPM2 was used to extract 600 bp dsRNA (ATU1) from cells bearing ATU1 construct and induced in media C. A i) uninduced cell, j) media C. B dsRNA extraction performed using DPM and eluted with 150 uL nuclease-free water with modifications as follows: k) NaCl solution adjusted to pH 6.0 (588.6 ng/uL, l) NaCl solution adjusted to pH 5.5 (215.8 ng/uL) m) NaCl solution adjusted to pH 5.5 (NH4)SO4 and SDS step performed together resulting in more prolonged incubation of sample in both, 480.3 ng/uL. n) NaCl solution adjusted to pH 5.0