Fig. 6
From: High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription
Transcription efficiency of systematically mutated initially transcribed regions of GFP gene quantified by STAR. The effect of substituting three other base pairs at each nucleotide position of 5′-UTR (from + 1 to + 8) in the DNA template was determined using STAR system. The X-axis represents different mutants, the Y-axis represents the relative fluorescence units. Error bars represent the standard deviation of the triplicate experiments, P < 0.05. The inset shows the results of denaturing polyacrylamide gel electrophoresis analysis of the 60-min transcription products