Table 1 Substrates and reaction conditions for enzymatic assay
Enzyme type | Substrate | Substrate brand |
|---|---|---|
Endo-β-1,4-glucanase | Carboxymethylcellulose, 1% (w/v) | Sinopharm, China |
Endo-β-1,4-xylanase | Glucuronoxylan, 1% (w/v) | Megazyme, Ireland |
Cellobiohydrolase | 4-Nitrophenyl-β-D-cellobioside, 10 mM | Chemsynlab, China |
β-Glucosidase | 4-Nitrophenyl β-D-glucopyranoside, 10 mM | Chemsynlab, China |
β-Xylosidase | 4-Nitrophenyl β-D-xylopyranoside, 10 mM | TCI, Japan |
α-L-Arabinofuranosidase | 4-Nitrophenyl α-L-arabinofuranoside, 10 mM | Chemsynlab, China |