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Table 5 The effects of different extracts exposure on the Caco-2 cells cell viability

From: Chemical composition and bioactivity of oilseed cake extracts obtained by subcritical and modified subcritical water

Sample

Control

Cell viability (%)

250 µg/mL

500 µg/mL

1000 µg/mL

Pumpkin 1

100.00 ± 9.41

97.17 ± 13.63b

119.95 ± 21.12a

115.95 ± 12.13a,1

Pumpkin 2

96.02 ± 18.26a

97.13 ± 16.46 a

61.93 ± 9.77b,2

Pumpkin 3

105.20 ± 13.38

114.75 ± 16.06

118.32 ± 19.591

Hemp 1

58.11 ± 8.02

67.43 ± 9.92

61.05 ± 11.051

Hemp 2

74.85 ± 13.53a

67.29 ± 12.37a

8.93 ± 1.17b,2

Hemp 3

82.21 ± 10.48

76.77 ± 15.42

92.19 ± 15.421

Flax 1

86.07 ± 16.761,2

76.40 ± 10.102

88.72 ± 18.131

Flax 2

85.18 ± 16.961

94.98 ± 19.771

96.10 ± 18.821

Flax 3

72.89 ± 10.67a,2

55.90 ± 7.88b,3

47.61 ± 10.30b,2

  1. Values are expressed as mean ± standard deviation (n = 6)
  2. Different letters (a, b, c) in the same row indicate significant differences between concentrations of the same sample extracted by different methodologies (p < 0.05), according to Tukey’s HSD test
  3. Different superscript numbers (1,2,3) in the same species at the same concentration represent significant differences (p < 0.05) between the extraction technique employed (p < 0.05), according to Tukey’s HSD test
  4. Subcritical water extraction carried out with a solid to solvent ratio of 1:30 (w/v), 1 h at 160 °C with (1) 20 bar N2, (2) 20 bar N2 with 0.05 M HCl, (3) 20 bar CO2