Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Bioresources and Bioprocessing

Table 1 The efficiency of Minos and Restless transposons in Geomyces sp. WNF-15A

Transposable systems	Minos		Restless
Transposable systems	Single	Dual	Single	Dual
The number of transformants	1041	830	339	579
The number of transposable mutants	101	99	39	76
The number of transposable mutants screened at 20 °C	97	95	37	72
The number of transposable mutants screened at 25 °C	4	4	2	4
The efficiency of transposition (%)	9.7	11.9	11.5	13.1
The number of positive mutants in red pigment^a	7	6	3	7
The number of positive mutants in growth^b	5	5	1	1
The positive mutation ratio (%)	11.9	11.1	10.3	10.5

^aThe mutants with variation percentage over 10% in red pigment production were considered as positive mutants, including MPS1–MPS10 and MPD1–MPD13
^bThe mutants with variation percentage over 15% in cell growth were considered as positive mutants, including MPS1, MPS3, MPS4, MGS1, MGS3, MGS6, MPD1, MPD2, MPD4, MGD2, MGD4, and MGD6