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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Fig. 2

A High-density fermentation culture of recombinant strains was carried out in a 5-L bioreactor. Glycerol feeding for some 4 h after 24th hour of inoculation and methanol was fed at 30th h after 2 h of starvation treatment. B SDS-PAGE analysis of broth supernatant after 36 h of inoculation. Lanes 1–9 were cultured 36, 42, 48, 54, 60, 66, 72, 78, and 84 h (loading volume 20 μL, not concentrated), respectively, lanes M1 and M2 were protein ladder. C Affinity purification of expression products. D The purified product exhibited a single band verified by SDS-PAGE. Lane 1 was supernatant of broth before purification, lane 2 was purified product, and lane M was standard molecular weights

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