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Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Fig. 1

Expression of RHLC via recombinant P. pastoris. a Sketch map of construction of recombinant P. pastoris GS115 with RHLC gene and followed expressed in shake flask and fermenter. b SDS–PAGE analysis of RHLC prepared by shake flask fermentation with different induction times and induction by methanol. c Wet cell mass (g/L) and protein content (g/L) of RHLC in a 5-L fermenter following induction by methanol. d SDS–PAGE analysis of RHLC in a 5-L fermenter with different induction times and induction by methanol

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