Fig. 2From: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastorisPurification process of RHLC and purity identification. a Purification process of RHLC fermentation broth. b SDS–PAGE of purified RHLC. Lane 1 was origin fermentation broth, lane 2 was purified RHLC, and lane 3 was redissolved of freeze-dried RHLC. c Different states of RHLC at different temperatures (Left was in ice bath and right was at room temperature). d Gel filtration analysis of redissolved freeze-dried RHLC samples, with 98% purityBack to article page