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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Fig. 3

Characteristic of RHLC and collagen sponge samples. a Circular dichroism (CD) spectra recorded at a wavelength of 190–260 nm. A significant negative peak at 198 nm indicating a triple helical structure. b DSC analysis of the thermal denaturation temperature of freeze-dried samples were heated from 40 °C to 200 °C at a rate of 5 °C/min. c Fourier transform infrared spectroscopy (FTIR) analysis at a wavelength range of 650–4000 cm-1 under ATR mode. d XRD analysis with the 2θ scanning range was from 5° to 80° with a scan speed of 5° per min. The collagen sponge has a lower 20° (2θ) peak, indicating slight degradation of the triple helical structure. e–j SEM analysis of freeze-dried RCHL and collagen sponge samples. The three adjacent panels represent the same samples. e Freeze-dried RHLC. f SEM image at a scale plate of 500 μm. g SEM image at a scale plate of 100 μm. h Freeze-dried collagen sponge. i SEM image at a scale plate of 500 μm. j SEM image at a scale plate of 100 μm

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