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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Fig. 1

Identification of the key sequence and engineering of cdna1 promoter. A Extracellular β-mannanase activities of strains expressing poman5A with different lengths of cdna1 promoter. The parent strain QP4 was used as a control. The samples were taken after 48 h of cultivation. B SDS-PAGE analysis of culture supernatants analyzed in panel A. C Schematic representation of the original (Pc1159) and engineered (PcMC) cdna1 promoter. D Extracellular β-mannanase activities of strains expressing poman5A with original and engineered cdna1 promoter. E SDS-PAGE analysis of the culture supernatants at 72 h analyzed in panel D. Data in panels A and D represent mean ± SD from triplicate cultivations

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