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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Fig. 2

Engineering of the 5′-untranslated region of cdna1 promoter. A Schematic representation of the 5′-UTR replaced promoters. Yellow and green boxes indicate 5′-UTRs from cel5A and cel7A promoters, respectively. The 123-bp intron in the UTR of cel5A is indicated by a thick line. B Extracellular β-mannanase activities of strains expressing poman5A with different promoters at 48 h. Data represent mean ± SD from triplicate cultivations. C SDS-PAGE analysis of the culture supernatants analyzed in panel B

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