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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Fig. 3

The effect of temperature on protein expression driven by cdna1 promoter. A β-Mannanase production with Pc1159 promoter under different conditions. The samples were taken after 24 h of cultivation. Data represent mean ± SD from duplicate cultivations. Xyl, xylose. Low P, low phosphate. B The effect of temperature on β-mannanase production of the strain expressing poman5A with Pc1159. The parent strain was used as a control. C SDS-PAGE analysis of the culture supernatants analyzed in panel B. D The effect of temperature on cell growth. E The effect of temperature on the transcript abundances of cdna1 and poman5A in the strain expressing poman5A with Pc1159. RNA samples were taken at 24 h. F The effect of temperature on endopolygalacturonase production by the strain expressing popga with Pc1159. G SDS-PAGE analysis of the culture supernatants at 48 h analyzed in panel F. Data in panels B and D–F represent mean ± SD from triplicate cultivations. In panel A, the statistical significances of the difference between control and cultures under different conditions are shown. In panels B and F, the statistical significances of the difference between cultures at 30 °C and 37 °C are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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