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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Fig. 5

Time courses showing DHA accumulation in fed-batch fermentations. Fermentation of engineered strain G. oxydans WSH-003–4 was conducted in 5 L bioreactor. A Initial glycerol addition was 100 g L − 1, and then 40 g L − 1 glycerol was fed at 40 h (Total glycerol addition was 140 g L − 1). B Initial glycerol addition was 120 g L − 1, and then 20 g L − 1 glycerol was fed at 42 h (Total glycerol addition was 140 g L − 1). C Initial glycerol addition was 120 g L − 1, and then glycerol was intermittently fed (20 g L − 1 each time) at 42 h, 48 h, 56 h, 66 h, 80 h, and 96 h (Total glycerol addition was 240 g L − 1). Black triangles; glycerol, blue squares; DHA, red circles; OD600

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