Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Bioresources and Bioprocessing

Table 2 Fermentation characteristics of engineered strains with knocked out dehydrogenase genes

Strains	OD₆₀₀	Production (g·L⁻¹)	Conversion (%)	Productivity (g·L⁻¹·h⁻¹)	Enhancement (%)
G. oxydans WSH-003	3.72	62.90	62.90	0.87	/
G. oxydans WSH-003–1	3.65	79.26	79.26	1.10	26.01
G. oxydans WSH-003–2	3.70	81.33	81.33	1.13	29.30
G. oxydans WSH-003–3	3.76	82.54	82.54	1.15	31.22
G. oxydans WSH-003–4	3.74	89.49	89.49	1.24	42.27
G. oxydans WSH-003–5	3.63	81.27	81.27	1.13	29.21
G. oxydans WSH-003–6	3.68	83.90	83.90	1.17	33.39
G. oxydans WSH-003–7	3.62	65.36	65.36	0.91	3.91
G. oxydans WSH-003–8	3.85	77.98	77.98	1.08	23.97
G. oxydans WSH-003–9	3.80	64.58	64.58	0.90	2.67
G. oxydans WSH-003–10	3.48	80.39	80.39	1.12	27.81
G. oxydans WSH-003–11	3.61	79.78	79.78	1.11	26.84
G. oxydans WSH-003–12	3.64	79.39	79.39	1.10	26.22
G. oxydans WSH-003–13	3.44	66.96	66.96	0.93	6.45
G. oxydans WSH-003–14	3.71	68.82	68.82	0.96	9.41
G. oxydans WSH-003–15	3.64	77.64	77.64	1.08	23.43
G. oxydans WSH-003–16	3.60	69.18	69.18	0.96	9.98

^*The OD₆₀₀ and DHA production were the mean value of triple parallel experiments