Point mutation of V252 in neomycin C epimerase enlarges substrate-binding pocket and improves neomycin B accumulation in Streptomyces fradiae

Bioresources and Bioprocessing

Table 2 Whole-genome sequencing of strain SF-2 with respect to neomycin biosynthesis pathway

Genes	Mutation base	Mutation amnio acid residue
NeoE	T91C, C112T, G895A	None
NeoS	G373A, C652T	None
NeoC	T412C, T958G, G1018T	None
NeoM	G247C, C585T, A1115G	V49A, A226T
NeoQ	C43G, C1366G	None
NeoN	G667A	None
NeoP	G241T, C261T, C448T, G518C	A62G, A148T
NeoX	G46C, C176T	C26Y
NeoF	T14C, C69T, A265C, G795T, C931T	A345T, E363G
NeoD	G108C, A205C,G332C, C470T, T501A, C832T	T113S, P170R, G124D, P245A
NeoL	G142C, T347C, T362C, G395C, C403G, G428A, C510G, T834G, C930T, A1485G, T1599C, A1602G, G1608C, T1659A, C1918G	E48Q, V116A, V121A, Q135E, R143H, P640A
NeoB	G61C, C475G, G1092C, G1219A	None
NeoA	T161C, G283C, A376T, G461A, G859C, T991C, C1111T, G1341C, G2099C, T2151C, G3396A, 2088 Del, 2113 Del, 2125 Del, 3649_3650 Indel CCCGGTGTT	P163S, T578A, R589V, G590A, S591V, P592A, A593R, W594L, T595E, R596A, P597A, R848G, A1141V, D1241G, 70_71 Indel GTP, 588 Del
NeoR	T876C, T995A, C1089G, G1272C, T1529C, C1617T, A1671G, T1989C, C2319G, C2331T, T2373C, G2374C, G2644A	V332D, T510I, G792R, E882K, 866_867 Indel LAAA
NeoG	G597C, C647T, G696A	A216V
NeoH	C81G	None
NeoI	None	None