Point mutation of V252 in neomycin C epimerase enlarges substrate-binding pocket and improves neomycin B accumulation in Streptomyces fradiae

Bioresources and Bioprocessing

Table 3 Information on the catalytic pocket of each mutant

Size and shape descriptors	Volume[Å³]	Surface[Å²]	Depth[Å]
NeoN	912.64	1370.45	22.03
NeoN^E34A	694.91	1053.27	19.94
NeoN^K36A	818.18	1498.79	21.50
NeoN^S251A	1349.63	1850.20	30.82
NeoN^V252A	963.65	1220.57	19.50