Adsorptive removal of levofloxacin and antibiotic resistance genes from hospital wastewater by nano-zero-valent iron and nano-copper using kinetic studies and response surface methodology

Bioresources and Bioprocessing

Table 6 Analysis of variance (ANOVA) for the response surface quadratic model for removal of the levofloxacin by (a) nZVI and (b) CuONPs

Source(a)	Sum of squares	df	Mean square	F-value	p-value
Model	2067.52	14	147.68	134.89	?<?0.0001	Significant
A-ph	647.83	1	647.83	591.72	?<?0.0001
B-con	123.39	1	123.39	112.71	?<?0.0001
C-dose	48.12	1	48.12	43.95	?<?0.0001
D-time	183.61	1	183.61	167.71	?<?0.0001
AB	7.90	1	7.90	7.21	0.0178
AC	0.2070	1	0.2070	0.1891	0.6703
AD	31.58	1	31.58	28.85	?<?0.0001
BC	3.52	1	3.52	3.21	0.0948
BD	21.86	1	21.86	19.96	0.0005
CD	7.70	1	7.70	7.03	0.0190
A²	897.99	1	897.99	820.22	?<?0.0001
B²	11.69	1	11.69	10.68	0.0056
C²	4.43	1	4.43	4.05	0.0639
D²	91.33	1	91.33	83.42	?<?0.0001
Residual	15.33	14	1.09
Lack of fit	14.08	10	1.41	4.51	0.0799	Not significant
Pure error	1.25	4	0.3123
Cor total	2082.85	28
Std. Dev	1.05			R²		0.9926
Mean	70.80			Adjusted R²		0.9853
C.V. %	1.48			Predicted R²		0.9601
				Adeq precision		39.3323

Source (b)	Sum of squares	df	Mean square	F-value	p-value
Model	3094.40	14	221.03	49.97	?<?0.0001	Significant
A-ph	1144.26	1	1144.26	258.67	?<?0.0001
B-con	599.25	1	599.25	135.47	?<?0.0001
C-dose	10.98	1	10.98	2.48	0.1374
D-time	357.52	1	357.52	80.82	?<?0.0001
AB	10.18	1	10.18	2.30	0.1516
AC	0.0156	1	0.0156	0.0035	0.9534
AD	0.0156	1	0.0156	0.0035	0.9534
BC	3.84	1	3.84	0.8684	0.3672
BD	0.5625	1	0.5625	0.1272	0.7267
CD	13.14	1	13.14	2.97	0.1068
A²	799.98	1	799.98	180.84	?<?0.0001
B²	87.74	1	87.74	19.84	0.0005
C²	3.60	1	3.60	0.8129	0.3825
D²	170.07	1	170.07	38.45	?<?0.0001
Residual	61.93	14	4.42
Lack of Fit	25.26	10	2.53	0.2755	0.9553	Not significant
Pure Error	36.67	4	9.17
Cor Total	3156.33	28
Std. Dev	2.10			R²		0.9804
Mean	68.12			Adjusted R²		0.9608
C.V. %	3.09			Predicted R²		0.9358
				Adeq precision		24.7624