Bioresources and Bioprocessing

Table 1 The kinetic parameters of MiXly, EcXly and its mutants (EcXly^N599A, EcXly^N599D and EcXly^N599G)

From: Dissecting the essential role of N-glycosylation in catalytic performance of xanthan lyase

Enzyme	K_m(μM)	K_cat(min⁻¹)	K_cat/K_m(μM⁻¹ min⁻¹)
MiXly^d	38.455	0.181	0.031
EcXly	18.752 ± 1.042^a	0.254 ± 0.016^a	0.014 ± 0.001^a
EcXly^N599A	1.361 ± 0.267^c	0.021 ± 0.003^c	0.016 ± 0.003^a
EcXly^N599D	8.414 ± 0.910^b	0.109 ± 0.009^b	0.013 ± 0.001^a
EcXly^N599G	1.314 ± 0.016^c	0.021 ± 0^c	0.015 ± 0^a

^a,b,cThe superscript different letters in the same column represent significant difference (P < 0.05), whereas the superscript same letter in the same column suggests no significant difference (P > 0.05)
^d The kinetic data were from Yang et al. (2014)

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