Fig. 1
From: Expression of recombinant human Apolipoprotein A-IMilano in Nicotiana tabacum
Construction of recombinant plasmid pCHF3–Flag–Apo A-IMilano, recombinant plasmid pCHF3–Apo A-IMilano–GFP and pCHF3–His6tag–GFP–TEV–Apo A-IMilano. The plant expression vector pCHF3, encoding a fusion protein consisting of 3 × Flag (GFP, His6tag–GFP) was fused in-frame with Apo A-IMilano. The coding sequence was under the transcriptional control of a CaMV 35S promoter