Fig. 7
From: Expression of recombinant human Apolipoprotein A-IMilano in Nicotiana tabacum
Representative of purification of Flag–Apo A-IMilano protein by SDS–PAGE gel. A Flag–Apo A-IMilano purified protein was separated by SDS–PAGE gel electrophoresis and stained with Coomassie brilliant blue. Lane M is standard protein molecular weight marker. Lane 1 presents purified Flag–Apo A-IMilano protein. B Image of the SDS–PAGE of Flag–Apo A-IMilano Purified Protein. C Quantification of the grayscale of the purified protein in the gel image with Image J. The ratio of the purified Flag–Apo A-IMilano to the total protein. The purity of Flag–Apo A-IMilano calculated by three experiments is 90.58% ± 1.65