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Fig. 12 | Bioresources and Bioprocessing

Fig. 12

From: Production of indigo by recombinant bacteria

Fig. 12

Sensitivity analysis chart, that presents percentage change in the annual output of indigo from a biological platform for scenarios. As the baseline (0%), the figure considers the anticipated product quantity (540 tonnes per annum), which has been calculated with the assumptions listed in Table 3., and based on the bioprocess sequence from Flowsheet A (Fig. 10). The light blue marks the best-case scenario, that could be achieved with short-to-medium-term process optimization, and the dark blue marks the predicted worst-case scenarios, where Table 3. values would not be achieved. For the value of upstream reaction titer, the worst-case scenario is 1 g/L, while the best-case scenario is 23 g/L. For the cumulative step yield, the former case is based on a sequence of 4 process steps, each of 80% yield, while the latter is based on the same number of steps, where each enables achieving 95% yield

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