Fig. 13

A graph that represents the projected annual output for a recombinant biological indigo platform depending on the alterations of 2 main values, that have been dictated by the Sensitivity Analysis presented in Fig. 12. The assumption is that platform relies on the bioprocess sequence from Flowsheet A (Fig. 10). In Fig. 12, the basic scenario has been fully based on Table 3. values, and equals 540 tonnes. Each graph represents a scenario, where a singular factor from Fig. 11 fluctuates—the reaction titer or the DSP step yields—or for their simultaneous alteration. The equation used for output calculations is the same as the equation that was used for Table 3. output estimation, which has been Output = (fermenter number) × (output from single fermenter per batch) × (batch runs/year) (Table 3.). The values used for calculations have been taken from Table 3. assumptions, unless stated otherwise. For example, the 2nd bar from the left was calculated with Table 3. inputs, except for DSP yield, which for calculation purposes has changed from 0.75% (top-down approach) total to 4 × 95% (bottom-up approach)