Fig. 2
From: Construction of the genetic switches in response to mannitol based on artificial MtlR box
The rational design for MtlR box. The rational mutations were introduced into eGFP expression cassette by overlap extension PCR. Six MtlR boxes mutants were designed to test the OFF-state value and ON-state value. The resultant mutations were subjected to parallel operation of OFF/ON under two conditions (1) with no inducer (2) + 1.5% mannitol. The eGFP fluorescence was test after added inducer 12 h