Fig. 3
From: Construction of the genetic switches in response to mannitol based on artificial MtlR box
The test of MtlR box in vitro. A Gene sequences of different MtlR boxes. The sequence in red represent the conserved sequence of the MtlR box, and those in green represent non conserved sequence. MtlR box 1: CCCGGGcacggctccTGCCAA; MtlR box 2: TTGTCAcacggctccCCCGGG; MtlR box 3:TTGTCATGCCAA; MtlR box 4: TTGTCAtccTGCCAA; MtlR box 5: TTGTCAggctccTGCCAA;MtlR box 6: TTGTCAcacggctccTGCCAA. B Six fragments (A1, A2, A3, A4, A5, A6), that carried MtlR box 1, 2, 3, 4, 5, 6, respectively, were selected as probes, EMSA was performed by MtlR protein in different concentration (0, 0.5 µM, 1.0 µM) for six fragments. C The dynamic ranges of six MtlR boxes were shown