Fig. 3
From: Large-scale expansion of human umbilical cord-derived mesenchymal stem cells using PLGA@PLL scaffold
Cell senescence was analyzed by β-galactosidase staining and RT-qPCR. A native hUC-MSCs from Wharton’s jelly and B hUC-MSCs released from PLGA@PLL scaffold. After β-galactosidase staining, all samples were observed under a microscope and displayed in three random regions. C Expression levels of the key cellular senescence genes P16 and P21 among the control and the scaffold groups were compared. The real-time PCR data were normalized to the internal control β-actin and plotted (n = 5). **indicates p < 0.01, two-tailed t-test Scale bar: 100 μm