Bioresources and Bioprocessing

Table 1 Asymmetric synthesis of THIQAs at different temperatures with benzaldehyde and dopamine as substrates

From: Engineering a norcoclaurine synthase for one-step synthesis of (S)-1-aryl-tetrahydroisoquinolines

Entry	°C	Conversion^[a] (%)			ee^[b] (%)
Entry	°C	Blank	WT	L68T/M97V	WT	L68T/M97V
1	25	6	35	84	85	95
2	30	10	53	96	97	98
3	40	11	56	97	92	92

The reaction mixture was composed of 50 mM amine, 25 mM aldehyde, 1 mg purified enzyme loaded with benzaldehyde, 20% (v/v) DMSO, 5 mM ascorbic acid and HEPES buffer (100 mM, pH 7.5)
All reactions were performed at 40 °C for 3 h, in duplicate ^[a][b], and analysed by HPLC

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