Table 2 Kinetic parameters of TfNCS and mutant L68T/M97V toward benzaldehyde and 4-biphenylaldehyde
From: Engineering a norcoclaurine synthase for one-step synthesis of (S)-1-aryl-tetrahydroisoquinolines
Enzyme | Aldehyde | kcat (s−1) | Km (mM) | kcat/Km (s–1 μM−1) |
|---|---|---|---|---|
WT | Benzaldehyde | 0.237 ± 0.028 | 22.3 ± 6.7 | 10.6 |
L68T/M97V | 0.439 ± 0.047 | 20.1 ± 5.3 | 21.8 | |
WT | 4-Biphenylaldehyde | 0.001 ± 0.0001 | 15.0 ± 4.6 | 0.06 |
L68T/M97V | 0.002 ± 0.0002 | 14.6 ± 5.1 | 0.14 |