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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction

Fig. 2

Engineering R. toruloides NP11 for MAN5C secretory expression. A Schematic of MAN5C expression cassette; B PCR result for MAN5C gene verification using the genomic DNA as templates; C Western blot analysis of the recombinant MAN5C. Lane M: Marker; Lane 1, 2: Represent samples from NP11-MAN5C and NP11, respectively; Lane + : The plasmid pZPK-PADH2-MAN5C-P2A-HYG-THSP; OC, IC: Represent the protein samples from the culture supernatant and cell lysates, respectively

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