Fig. 2From: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extractionEngineering R. toruloides NP11 for MAN5C secretory expression. A Schematic of MAN5C expression cassette; B PCR result for MAN5C gene verification using the genomic DNA as templates; C Western blot analysis of the recombinant MAN5C. Lane M: Marker; Lane 1, 2: Represent samples from NP11-MAN5C and NP11, respectively; Lane + : The plasmid pZPK-PADH2-MAN5C-P2A-HYG-THSP; OC, IC: Represent the protein samples from the culture supernatant and cell lysates, respectivelyBack to article page