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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction

Fig. 3

Results of cell growth and MAN5C production. A Cell growth profiles on YEPD agar plate and that supplemented with Congo red; B Cell growth profiles in YEPD media at 30 ℃, 800 rpm in a microbial growth analysis system; C Profiles of glucose consumption and cell growth in nitrogen-limited media at 30 ℃, 200 rpm; D Western blot results of the supernatants of cell lysates; E SDS-PAGE results of the culture broth. Lane M: Marker; Lanes 1 to 5 were samples from the culture in nitrogen-limited media at 24, 48, 72, 96, and 120 h, respectively; Lane + : Mature MAN5C expressed by P. pastoris X-33

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