Table 1 Strains and plasmids used in this study
Strains or plasmids | Genotype or characteristic | Resource |
|---|---|---|
Strains | ||
4# | R. toruloides CGMCC 2.1389 | CGMCC |
NP11 | R. toruloides GDMCC 2.224, MAT A1 | (Zhu et al. 2012) |
Escherichia coli DH10B | F- endA1 deoR recA1 galE15 galK16 nupG, rpsLΔ(lac)X74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) StrR λ− | Invitrogen |
Agrobacterium tumefaciens AGL1 | AGL0 recA::bla pTiBo542DT Mop + CbR | (Lin et al. 2014) |
P. pastoris pPICZαA-plman5c | P. pastoris X-33 carrying the plasmid pPICZαA-plman5c | (Yang et al. 2011) |
NP11-MAN5C | NP11 carrying the PADH2-MAN5C-P2A-HYG-THSP expression cassette | This study |
Plasmid | ||
pZPK-PADH2 | PADH2 in pZPK | Lab collection |
pZPK-PPGK-MNP-P2A-HYG-THSP | PPGK-MNP-P2A-HYG-THSP in pZPK | (Lyu et al. 2021) |
pUC-MAN5C | MAN5C in pUC57 | This study |
pZPK-PPGK-MAN5C-P2A-HYG-THSP | PPGK-MAN5C-P2A-HYG-THSP in pZPK | This study |
pZPK-PADH2-MAN5C-P2A-HYG-THSP | PADH2-MAN5C-P2A-HYG-THSP in pZPK | This study |