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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris

Fig. 3

Engineering of variant N181A. a The overall structure of CtPL-DM (PDB ID, 8IAN) in cartoon representation. Residues selected for engineering are displayed in sticks, with each color correspond to the mutation strategies indicated in the colored box. The residues comprising the catalytic triad (green box) are shown as sticks. b De-glycosylated P. pastoris-expressed recombinant protein of each variant were incubated with GfPET film at indicated temperature for 18 h. The total amount of hydrolytic products (MHET and TPA) were measured and presented as shown

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