Skip to main content
Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Fig. 2

Construction of a 3-HP sub-pathway in S. cerevisiae. A Part of the 3-HP sub-pathway from propionate and glyoxylate. B GC–MS analysis of mesaconate produced from propionate and glyoxylate in engineered yeasts with various MCHs from different species. Strains were cultured with SC-dropout medium with 0.1% propionate and 0.1% glyoxylate. M01: CEN.PK 2-1D with the expression of SePrpE, RsMCL and EcYciA; M01-C: M01 with empty plasmid; M01-CaMCH: M01 with the expression of CaMCH; M01-RsMCH: M01 with the expression of RsMCH; M01-HmMCH: M01 with the expression of HmMCH. Mesaconate, the standard of mesaconate at 30 μg/L. C The yield of the reporter mesaconate from glucose. M201, strain M2 harboring the plasmid pSC-HIS-MCR for overexpressing MCR; M202, strain M2 harboring the plasmid pSC-HIS-MCR-YciA for overexpressing MCR and YciA. Strains were cultured with SC-dropout medium with 0.1% glyoxylate

Back to article page