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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Fig. 3

Functional validation of endogenous acyl-CoA hydrolases/transferases in yeast. A Production of mesaconate in yeast M3 with the overexpression of ACH1, EHD3 and TES1. M3-TRP: M3 harboring the empty plasmid pSC-TRP; M3-ACH1: M3 harboring the plasmid pSC-TRP-ACH1 for overexpressing ACH1; M3-EHD3: M3 harboring the plasmid pSC-TRP-EHD3 for overexpressing EHD3; M3-TES1: M3 harboring the plasmid pSC-TRP-TES1 for overexpressing TES1. B Production of mesaconate in yeast M3 with the knockout of ACH1, EHD3 and TES1; M3-ΔACH1: deletion of ACH1 in M3; M3-ΔEHD3: deletion of EHD3 in M3; M3-ΔTES1: deletion of TES1 in M3. Strains were cultured with SC-dropout medium with 0.1% propionate and 0.1% glyoxylate

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