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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Fig. 5

Optimization of the 3-HP sub-pathway in S. cerevisiae. A Evaluation of different 3-hydroxypropionyl-CoA synthases in the 3-HP sub-pathway. M101: strain M1 harboring the plasmid pSC-HIS-MsHPCS-MsACR-StHPCD and pUGG-PH for overexpressing MsHPCS, MsACR, StHPCD and MCR; M102: M1 harboring the plasmid pSC-HIS-SePrpE-MsACR-StHPCD and pUGG-PH for overexpressing SePrpE, MsACR, StHPCD and MCR; M103, M102 with an additional copy of SePrpE expression cassette in XII-1 site. B The yield of the reporter mesaconate from glucose. M201, strain M2 harboring the plasmid pSC-HIS-MCR for overexpressing MCR; M203, strain M2 harboring the plasmid pUGG-PH for overexpressing MCR; M403, the SePrpE expression cassette replaced the MsHPCS expression cassette at the XI-2 site of M203, and an additional copy of the SePrpE expression cassette was integrated into the XII-1 site; M405, strain M403 with additional copy of SePrpE in the plasmid pUGG-PH. Strains were cultured with SC-dropout medium with 0.1% glyoxylate

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