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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Fig. 6

Optimization of the 3-HP sub-pathway in S. cerevisiae with ethanol as the carbon source. A 3-HP production in engineered yeast from ethanol by expression of MCR-C and MCR-N under control by different promoter combinations. B The production of the reporter mesaconate in engineered yeasts with ethanol as the carbon source. M201, strain M2 harboring the plasmid pSC-HIS-MCR for overexpressing MCR; M204, strain M2 harboring the plasmid pUGG-TEQ for overexpressing MCR; M404, the SePrpE expression cassette replaced the MsHPCS expression cassette at the XI-2 site of M204, and an additional copy of the SePrpE expression cassette was integrated into the XII-1 site; M406, strain M404 with additional copy of SePrpE in the plasmid pUGG-TEQ

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