Articles

Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production

Microbial organelles are a promising model to promote cellular functions for the production of high-value chemicals. However, the concentrations of enzymes and nanoparticles are limited by the contact surface ...

Authors: Qiang Ding, Yadi Liu, Guipeng Hu, Liang Guo, Cong Gao, Xiulai Chen, Wei Chen, Jian Chen and Liming Liu

In vitro biosynthesis of ATP from adenosine and polyphosphate

Adenosine triphosphate (ATP) acts as a crucial energy currency in vivo, and it is a widely used energy and/or phosphate donor for enzyme-catalyzed reactions in vitro. In this study, we established an in vitro mul...

Authors: Chuanqi Sun, Zonglin Li, Xiao Ning, Wentian Xu and Zhimin Li

Production of bacterial cellulose from glycerol: the current state and perspectives

Current research in industrial microbiology and biotechnology focuses on the production of biodegradable microbial polymers as an environmentally friendly alternative to the still dominant fossil hydrocarbon-b...

Authors: Peteris Zikmanis, Sergejs Kolesovs, Maija Ruklisha and Pavels Semjonovs

A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Although Escherichia coli has been widely used for the expression of exogenous proteins, the secretory expression in this system is still a big obstacle. As one of the most important secretion pathways, hemolysin...

Authors: Wen Zhu, Lifu Hu, Yang Wang, Liangyin Lv, Hui Wang, Wenqiang Shi, Jianwei Zhu and Huili Lu

Kinetic modelling of the solid–liquid extraction process of polyphenolic compounds from apple pomace: influence of solvent composition and temperature

This study aims to assess kinetic modelling of the solid–liquid extraction process of total polyphenolic compounds (TPC) from apple pomace (AP). In this regard, we investigated the effects of temperature and s...

Authors: Parinaz Hobbi, Oseweuba Valentine Okoro, Christine Delporte, Houman Alimoradi, Daria Podstawczyk, Lei Nie, Katrien V. Bernaerts and Amin Shavandi

Valorisation of corncob into furfuryl alcohol and furoic acid via chemoenzymatic cascade catalysis

Heterogeneous tin-based sulfonated graphite (Sn-GP) catalyst was prepared with graphite as carrier. The physicochemical properties of Sn-GP were captured by FT-IR, XRD, SEM and BET. Organic acids with differen...

Authors: Jiacheng Ni, Junhua Di, Cuiluan Ma and Yu-Cai He

A novel fragmented anode biofilm microbial fuel cell (FAB–MFC) integrated system for domestic wastewater treatment and bioelectricity generation

The critical MFC design challenge is to increase anode surface area. A novel FAB–MFC integrated system was developed and evaluated for domestic wastewater treatment. It was operated in fed-batch flow mode at 1...

Authors: Tesfalem Atnafu and Seyoum Leta

Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis

The biotechnological production of the carotenoid astaxanthin is done with the microalgae Haematococcus pluvialis (H. pluvialis). Under nutrient deficiency and light stress, H. pluvialis accumulates astaxanthin i...

Authors: Andreas Bauer and Mirjana Minceva

Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast

Steroidal compounds are of great interest in the pharmaceutical field, with steroidal drugs as the second largest category of medicine in the world. Advances in synthetic biology and metabolic engineering have...

Authors: Yuehao Gu, Xue Jiao, Lidan Ye and Hongwei Yu

Customized exogenous ferredoxin functions as an efficient electron carrier

Ferredoxin (Fdx) is regarded as the main electron carrier in biological electron transfer and acts as an electron donor in metabolic pathways of many organisms. Here, we screened a self-sufficient P450-derived...

Authors: Zhan Song, Cancan Wei, Chao Li, Xin Gao, Shuhong Mao, Fuping Lu and Hui-Min Qin

Tyrosinase@HKUST-1: a super stable biocatalyst efficient for catecholic product synthesis

Although metal–organic frameworks (MOFs) have been considered as promising matrices for enzyme immobilization, HKUST-1, constructed from copper acetate (CuAc₂) and benzene 1,3,5-tricarboxylate (BTC), has rarely b...

Authors: Xiao-Feng Lü, Chao-Yun Feng, Shuangfei Li, Guo-Hao Liu and Zhen Yang

From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

The filamentous fungus Trichoderma reesei has been widely used for cellulase production that has extensive applications in green and sustainable development. Increasing costs and depletion of fossil fuels provoke...

Authors: Su Yan, Yan Xu and Xiao-Wei Yu

Recent advances in the microbial synthesis of lactate-based copolymer

Due to the increasing environmental pollution of un-degradable plastics and the consumption of non-renewable resources, more attention has been attracted by new bio-degradable/based polymers produced from rene...

Authors: Pengye Guo, Yuanchan Luo, Ju Wu and Hui Wu

Mutations in adaptively evolved Escherichia coli LGE2 facilitated the cost-effective upgrading of undetoxified bio-oil to bioethanol fuel

Levoglucosan is a promising sugar present in the lignocellulose pyrolysis bio-oil, which is a renewable and environment-friendly source for various value-added productions. Although many microbial catalysts ha...

Authors: Dongdong Chang, Cong Wang, Fabrice Ndayisenga and Zhisheng Yu

Quantitative analysis on photon numbers received per cell for triggering β-carotene accumulation in Dunaliella salina

Accumulation of β-carotene in Dunaliella salina is highly dependent on light exposure intensity and duration, but quantitative analysis on photon numbers received per cell for triggering β-carotene accumulation i...

Authors: Yimei Xi, Song Xue, Xupeng Cao, Zhanyou Chi and Jinghan Wang

Structure-guided protein engineering of ammonia lyase for efficient synthesis of sterically bulky unnatural amino acids

Enzymatic asymmetric amination addition is seen as a promising approach for synthesizing amine derivatives, especially unnatural amino acids, which are valuable precursors to fine chemicals and drugs. Despite ...

Authors: Zi-Fu Ni, Pei Xu, Min-Hua Zong and Wen-Yong Lou

Identification and structural analysis of a thermophilic β-1,3-glucanase from compost

β-1,3-glucanase can specifically hydrolyze glucans to oligosaccharides and has potential applications in biotechnology. We used the metatranscriptomic technology to discover a thermophilic β-1,3-glucanase from...

Authors: Jianwei Feng, Shenyuan Xu, Ruirui Feng, Andrey Kovalevsky, Xia Zhang, Dongyang Liu and Qun Wan

Novel cattail fiber composites: converting waste biomass into reinforcement for composites

Vacuum-assisted resin transfer molding (VARTM), used in manufacturing medium to large-sized composites for transportation industries, requires non-woven mats. While non-woven glass mats used in these applicati...

Authors: Md. Shadhin, Mashiur Rahman, Raghavan Jayaraman and Danny Mann

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

5-Aminolevulinic acid (5-ALA), a non-proteinogenic five-carbon amino acid, has received intensive attentions in medicine due to its approval by the US Food and Drug Administration (FDA) for cancer diagnosis an...

Authors: Ying-Chen Yi, I-Tai Shih, Tzu-Hsuan Yu, Yen-Ju Lee and I-Son Ng

Rice husk hydrochars from metal chloride-assisted hydrothermal carbonization as biosorbents of organics from aqueous solution

Hydrochar a carbon-rich material resulting from hydrothermal carbonization of biomass, has received substantial attention because of its potential application in various areas such as carbon sequestration, bio...

Authors: Yin Li, Fana Mulugeta Hagos, Rongrong Chen, Hanxin Qian, Chengxing Mo, Jing Di, Xikun Gai, Ruiqin Yang, Genxing Pan and Shengdao Shan

Polyhydroxyalkanoate production from rice straw hydrolysate obtained by alkaline pretreatment and enzymatic hydrolysis using Bacillus strains isolated from decomposing straw

Rice straw is an important low-cost feedstock for bio-based economy. This report presents a study in which rice straw was used both as a source for isolation of bacteria producing the biodegradable polyester p...

Authors: Doan Van Thuoc, Nguyen Thi Chung and Rajni Hatti-Kaul

One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Chiral phenylglycinol is a very important chemical in the pharmaceutical manufacturing. Current methods for synthesis of chiral phenylglycinol often suffered from unsatisfied selectivity, low product yield and...

Authors: Jiandong Zhang, Ning Qi, Lili Gao, Jing Li, Chaofeng Zhang and Honghong Chang

Application of near-infrared spectroscopy technology in the complex fermentation system to achieve high-efficiency production

The fermentation process is dynamically changing, and the metabolic status can be grasped through real-time monitoring of environmental parameters. In this study, a real-time and on-line monitoring experiment ...

Authors: Chen Yang, Chen Lingli, Guo Meijin, Li Xu, Liu jinsong, Liu Xiaofeng, Chen Zhongbing, Tian Xiaojun, Zheng Haoyue, Tian Xiwei, Chu Ju and Zhuang Yingping

Current perspective on production and applications of microbial cellulases: a review

The potential of cellulolytic enzymes has been widely studied and explored for bioconversion processes and plays a key role in various industrial applications. Cellulase, a key enzyme for cellulose-rich waste ...

Authors: Nisha Bhardwaj, Bikash Kumar, Komal Agrawal and Pradeep Verma

Biosynthesis of lactones from diols mediated by an artificial flavin

Lactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)⁺-dependent alcohol dehydrogenases-catalyzed oxidative...

Authors: Xiaowang Zhang, Zhuotao Tan, Chaojian Li, Siyu Qi, Mengjiao Xu, Ming Li, Wenlong Xiong, Wei Zhuang, Dong Liu, Chenjie Zhu and Hanjie Ying

Applications and analysis of hydrolysates in animal cell culture

Animal cells are used in the manufacturing of complex biotherapeutic products since the 1980s. From its initial uses in biological research to its current importance in the biopharmaceutical industry, many typ...

Authors: Yin Ying Ho, Hao Kim Lu, Zhi Feng Sherman Lim, Hao Wei Lim, Ying Swan Ho and Say Kong Ng

Biological pretreatment of corn stover for enhancing enzymatic hydrolysis using Bacillus sp. P3

The biological pretreatment for the enzymatic hydrolysis of lignocellulosic biomasses depends exclusively on the effective pretreatment process. Herein, we report a significant enhancement of enzymatic sacchar...

Authors: Yanwen Wu, Haipeng Guo, Md. Shafiqur Rahman, Xuantong Chen, Jinchi Zhang, Yun Liu and Wensheng Qin

Advances in engineering microbial biosynthesis of aromatic compounds and related compounds

Aromatic compounds have broad applications and have been the target of biosynthetic processes for several decades. New biomolecular engineering strategies have been applied to improve production of aromatic co...

Authors: Roman M. Dickey, Amanda M. Forti and Aditya M. Kunjapur

A novel decrystallizing protein CxEXL22 from Arthrobotrys sp. CX1 capable of synergistically hydrolyzing cellulose with cellulases

A novel expansin-like protein (CxEXL22) has been identified and characterized from newly isolated Arthrobotrys sp. CX1 that can cause cellulose decrystallization. Unlike previously reported expansin-like proteins...

Authors: Rong Li, Yunze Sun, Yihao Zhou, Jiawei Gai, Linlu You, Fan Yang, Wenzhu Tang and Xianzhen Li

Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine

β-Alanine (3-aminopropionic acid) is the only naturally occurring β-amino acid and an important precursor for the synthesis of a variety of nitrogen-containing chemicals. Fermentative production of β-alanine f...

Authors: Liangtian Miao, Yin Li and Taicheng Zhu

Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

Glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) as an important derivative of glycyrrhizin (GL) shows stronger biological activities and higher sweetness than GL. The biotransformation process is considered as ...

Authors: Boliang Gao, Yiwen Xiao, Qian Zhang, Junru Sun, Zhibing Zhang and Du Zhu

Sugarcane bagasse: a biomass sufficiently applied for improving global energy, environment and economic sustainability

Sugarcane (Saccharum officinarum) bagasse (SCB) is a biomass of agricultural waste obtained from sugarcane processing that has been found in abundance globally. Due to its abundance in nature, researchers have be...

Authors: E. O. Ajala, J. O. Ighalo, M. A. Ajala, A. G. Adeniyi and A. M. Ayanshola

Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO₂ capture

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key CO₂-fixing enzyme in photosynthesis, is notorious for its low carboxylation. We report a highly active and assembly-competent Form II Rubisco fro...

Authors: Junli Zhang, Guoxia Liu, Alonso I. Carvajal, Robert H. Wilson, Zhen Cai and Yin Li

Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A

Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfru...

Authors: Most Sheauly Khatun, Morteza Hassanpour, Solange I. Mussatto, Mark D. Harrison, Robert E. Speight, Ian M. O’Hara and Zhanying Zhang

Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae

In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With the exogenous addition of 0.3% (w/v) SLs in the initial m...

Authors: Qianqian Zhang, Zhiyue Xiong, Lei Sun, Xiwei Tian, Guiwei Tian, Yiming Yang, Xu Li, Yonghong Wang and Ju Chu

Correction to: Biocompatible natural deep eutectic solvent‑based extraction and cellulolytic enzyme‑mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health‑bioactive constituents

Authors: Fonthip Makkliang, Boondaree Siriwarin, Gorawit Yusakul, Suppalak Phaisan, Attapon Sakdamas, Natthapon Chuphol, Waraporn Putalun and Seiichi Sakamoto

Enhancement of co-production of lutein and protein in Chlorella sorokiniana FZU60 using different bioprocess operation strategies

Co-production of multiple compounds is an efficient approach to enhance the economic feasibility of microalgae-based metabolites production. In this study, Chlorella sorokiniana FZU60 was cultivated under differe...

Authors: Ruijuan Ma, Zhen Zhang, Zhuzhen Tang, Shih-Hsin Ho, Xinguo Shi, Lemian Liu, Youping Xie and Jianfeng Chen

Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Prochiral pyrmetazole can be asymmetrically oxidized into (S)-omeprazole, a proton pump inhibitor that is used to treat gastroesophageal reflux, by an engineered cyclohexanone monooxygenase (CHMO_Acineto-Mut) that...

Authors: Ya-Jing Li, Yu-Cong Zheng, Qiang Geng, Feng Liu, Zhi-Jun Zhang, Jian-He Xu and Hui-Lei Yu

Chemoenzymatic access to enantiopure N-containing furfuryl alcohol from chitin-derived N-acetyl-D-glucosamine

Chiral furfuryl alcohols are important precursors for the synthesis of valuable functionalized pyranones such as the rare sugar L-rednose. However, the synthesis of enantiopure chiral biobased furfuryl alcohol...

Authors: Ya-Cheng Hao, Min-Hua Zong, Zhi-Lin Wang and Ning Li

Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases

Pectate lyases and pectin lyases have essential roles in various biotechnological applications, such as textile industry, paper making, pectic wastewater pretreatment, juice clarification and oil extraction. T...

Authors: Ling Zheng, Yinxiao Xu, Qian Li and Benwei Zhu

α-Farnesene production from lipid by engineered Yarrowia lipolytica

Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well as improve the economic value of wasted oils and fats. Yarrowia lipo...

Authors: Yinghang Liu, Zhaoxuan Wang, Zhiyong Cui, Qingsheng Qi and Jin Hou

A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

To develop an economically feasible fermentation process, this study designed a novel bioprocess based on the co-culture of engineered Bacillus subtilis and Escherichia coli for the co-production of extracellular...

Authors: Jun Zhang, Wen Luo, Zhiyuan Wang, Xiaoyan Chen, Pengmei Lv and Jingliang Xu

Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents

The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus limiting their health benefits. Cellulolytic enzyme-assisted e...

Authors: Fonthip Makkliang, Boondaree Siriwarin, Gorawit Yusakul, Suppalak Phaisan, Attapon Sakdamas, Natthapon Chuphol, Waraporn Putalun and Seiichi Sakamoto

Densification of agro-residues for sustainable energy generation: an overview

The global demand for sustainable energy is increasing due to urbanization, industrialization, population, and developmental growth. Transforming the large quantities of biomass resources such as agro-residues...

Authors: Segun E. Ibitoye, Tien-Chien Jen, Rasheedat M. Mahamood and Esther T. Akinlabi

A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

The scale-up of animal cell cultivation is important but remains complex and challenging. In the present study, we propose a novel scale-up strategy for baby hamster Syrian kidney-21 (BHK-21) cell cultivation ...

Authors: Xiaonuo Teng, Chao Li, Xiaoping Yi and Yingping Zhuang

Physiological and transcriptome analysis elucidates the metabolic mechanism of versatile Porphyridium purpureum under nitrogen deprivation for exopolysaccharides accumulation

Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibited the growth of P. purpureum and resulted ...

Authors: Liang Ji, Shaohua Li, Cheng Chen, Haojie Jin, Haizhen Wu and Jianhua Fan

Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification

In the biosynthesis of natural products, methylation is a common and essential transformation to alter molecules’ bioavailability and bioactivity. The main methylation reaction is performed by S-adenosylmethionin...

Authors: Congqiang Zhang, Stella Amelia Sultan, Rehka T and Xixian Chen

Microbial lipid fermentation of Trichosporon cutaneum in high saline water

Fermentative production of microbial lipid requires high fresh water input. The utilization of high saline seawater or industrial wastewater is an important alternative to reduce the freshwater consumption. Th...

Authors: Luhuan Sun, Shuai Shao and Jie Bao

Ferulic acid production by metabolically engineered Escherichia coli

Ferulic acid (p-hydroxy-3-methoxycinnamic acid, FA) is a natural active substance present in plant cell walls, with antioxidant, anticancer, antithrombotic and other properties; it is widely used in medicine, foo...

Authors: Huajun Lv, Ying Zhang, Jie Shao, Haili Liu and Yong Wang

Conversion of lignocellulosic waste into effective flocculants: synthesis, characterization, and performance

Development of cationic flocculants from lignocellulosic wastes not only eliminates the health and environmental concerns associated with the use of conventional chemicals, but also is the way of waste valoriz...

Authors: Elham Jahedi and Reza Panahi