Articles

Combined ¹³C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, en...

Authors: Ping Liu, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang and Siliang Zhang

Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

Streptomyces aureofaciens strain A6-9, obtained with traditional mutagenesis, produces elevated levels of 6-DCT. The increased formation of 6-DCT may be attributable to the changes in ...

Authors: Hong Li, Ruifang Ye, Guizhen Lin, Deyu Zhu and QuanGui Mao

Improvement of chaetominine production by tryptophan feeding and medium optimization in submerged fermentation of Aspergillus fumigatus CY018

Chaetominine (CHA) is a novel alkaloid with excellent medicinal activities produced by Aspergillus fumigatus CY018. However, its further application has been severely restricted by the low production yield. In th...

Authors: Yu-peng Zhang, Rui-hua Jiao, Yan-hua Lu and Ling-yun Yao

Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the gener...

Authors: Zhoutong Sun, Pamela Torres Salas, Elina Siirola, Richard Lonsdale and Manfred T. Reetz

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

The organic nitrogen source is one of the key factors affecting Mortierella alpina cell growth as well as arachidonic acid (ARA) production. The aim of the present work is to achieve an optimized recipe of organi...

Authors: Xue-ping Ling, Si-yu Zeng, Cui-xue Chen, Xiao-ting Liu and Ying-hua Lu

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural c...

Authors: Pallavi Singh and Bhupendra N. Tiwary

Preliminary integrated economic and environmental analysis of polyhydroxyalkanoates (PHAs) biosynthesis

Being biodegradable and renewable, polyhydroxyalkanoates (PHAs), a green polymer, attract much attentions as potential alternative for conventional plastics due to increased concern towards environmental issue...

Authors: Yoong Kit Leong, Pau Loke Show, Heng Chou Lin, Chih Kai Chang, Hwei-San Loh, John Chi-Wei Lan and Tau Chuan Ling

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness. Hydrocarbon-polluted soils h...

Authors: Ijeoma Vivian Nwaguma, Chioma Blaise Chikere and Gideon Chijioke Okpokwasili

Effects of hot-washing process on structure and enzymatic hydrolysis of treated steam explosion corn stover

Cellulase adsorption of lignocellulosic materials is the key link during enzymatic hydrolysis. Hot-washing process (above lignin glass transition temperature) was used to change the physical structure of ligni...

Authors: Xu Yang, Xiaojian Ma, Hongliang Li, Junyin Chen and Shuqi Fang

Microbial fuel cell: a green approach for the utilization of waste for the generation of bioelectricity

Today we are witnessing a global energy crisis due to huge energy demands and limited resources. Non-renewable energy sources are depleting and renewable energy sources are not properly utilized. There is an i...

Authors: Venkatesh Chaturvedi and Pradeep Verma

Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system

Porcine circovirus type 2 (PCV2) virus-like particles (VLPs) are an effective vaccine against post-weaning multisystemic wasting syndrome. Burst sequence (BS) and the chicken 5′-HS4 β-globin (HS4) insulator we...

Authors: Yingjun Li, Xiaoping Yi, Yingping Zhuang and Ju Chu

Erratum to: Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditions

Authors: James W. Lee, Bob Hawkins, Michelle K. Kidder, Barbara R. Evans, Sokwon Paik, A. C. Buchanan and Danny Day

Cultivation of Nannochloropsis sp. using narrow beam angle light emitting diode in an internally illuminated photobioreactor

This paper reports on the growth condition of Nannochloropsis sp. in an annular column-type photobioreactor (PBR) using light-emitting diode as an internal illumination.

Authors: Mohamad Taisir, Chee Loong Teo, Ani Idris and Affendi M. Yusuf

Investigating the mechanism of nanofiltration separation of glucosamine hydrochloride and N-acetyl glucosamine

Glucosamine hydrochloride (GAH) and N-acetyl glucosamine (NAG) are chitin derivatives. Owing to their excellent biological activity, they have long been used as pharmaceuticals and nutraceuticals. However, both o...

Authors: Shengnan Zhang, Jiachun Zhou, Liqiang Fan, Yongjun Qiu, Lihua Jiang and Liming Zhao

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

This study investigated the effect of aeration rate and light intensity on biomass production and total fatty acids (TFA) accumulation by Porphyridium purpureum. The red microalgae is also known to accumulate con...

Authors: Gaomin Su, Kailin Jiao, Jingyu Chang, Zheng Li, Xiaoyi Guo, Yong Sun, Xianhai Zeng, Yinghua Lu and Lu Lin

Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa

The FadR subfamily of regulators plays essential roles in the regulation of diverse metabolic pathways in bacteria. LldR, an FadR-type regulator, regulates lactate utilization in Pseudomonas aeruginosa.

Authors: Bo Xin, Geng Wu, Kunzhi Zhang, Yongxing He, Hongzhi Tang, Chao Gao, Ping Xu and Cuiqing Ma

Synthesis and characterization of crystalline carboxymethylated lignin–TEOS nanocomposites for metal adsorption and antibacterial activity

Biodegradable carboxymethylated lignin–tetra ethoxysilane (TEOS) nanocomposites (CML–T) were synthesized using lignin extracted from rice straw (RS) followed by surface modification through carboxymethylation....

Authors: Kumari Shweta and Harit Jha

Optimization of milk-clotting enzyme production by Bacillus amyloliquefaciens SP1 isolated from apple rhizosphere

Present study aims to isolate and optimize fermentation conditions of milk-clotting enzyme producing rhizobacteria Bacillus amyloliquefaciens SP1.

Authors: Shiwani Guleria, Abhishek Walia, Anjali Chauhan and C. K. Shirkot

Lactic acid production from paper sludge by SSF with thermotolerant Rhizopus sp.

Rhizopus fungi is suitable for the production of lactic acid, which is the backbone material of polylactic acid used as green plastic from lignocellulosic biomass, since it can grow an...

Authors: Maki Takano and Kazuhiro Hoshino

Current status of cow dung as a bioresource for sustainable development

Cow dung, an excreta of bovine animal, is a cheap and easily available bioresource on our planet. Many traditional uses of cow dung such as burning as fuel, mosquito repellent and as cleansing agent are alread...

Authors: Kartikey Kumar Gupta, Kamal Rai Aneja and Deepanshu Rana

Synthesis and characterization of Acacia lignin-gelatin film for its possible application in food packaging

The aim of the present investigation was to develop Acacia lignin-gelatin (LG) blended films using glycerol as plasticizer and to establish correlation between lignin contents and structure, thermal and mechanica...

Authors: Keshaw Ram Aadil, Anand Barapatre and Harit Jha

Production of bioethanol by bacterial co-culture from agro-waste-impacted soil through simultaneous saccharification and co-fermentation of steam-exploded bagasse

The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agro-waste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bag...

Authors: Francis Sopuruchukwu Ire, Victor Ezebuiro and Chimezie Jason Ogugbue

Palm kernel oil-based polyester polyurethane composites incorporated with multi-walled carbon nanotubes for biomedical application

In this study, polyurethane (PU) films from palm kernel oil-based polyester (PKO-p) incorporated multi-walled carbon nanotubes (MWNTs) were prepared via evaporative casting method. Nanoparticle fluid dispersio...

Authors: Nurul Nabilah bt Zulkifli, Khairiah bt Hj Badri and Khairul Anuar Mat Amin

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable int...

Authors: Agus Sukito and Sanro Tachibana

Conversion of residues and by-products from the biodiesel industry into value-added products

Biodiesel, one of the most important sources of renewable energy, is produced in large quantities around the world; however, its production generates different kinds of residues and by-products which raise eco...

Authors: Jersson Plácido and Sergio Capareda

The synthesis of biodiesel catalyzed by Mucor miehei lipase immobilized onto aminated polyethersulfone membranes

Nowadays, biodiesel has become more attractive in the quest for a novel fuel source due to its environmental benefits and the biodegradable properties. Transesterification of Helianthus annuus (sunflower) seeds o...

Authors: Nurrahmi Handayani, Deana Wahyuningrum, Muhammad Ali Zulfikar, Santi Nurbaiti, Cynthia L. Radiman and Buchari

Characterization of a novel metallocarboxypeptidase from Streptomyces cinnamoneus TH-2

Carboxypeptidases are exopeptidases that catalyze the release of amino acids from the C-terminus of peptides or proteins. The peptides consisting of hydrophobic amino acids taste bitter. Therefore, the hydroly...

Authors: Kun Wan, Misugi Uraji, Jiro Arima and Tadashi Hatanaka

Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

Palm oil mill effluent (POME) generated from the palm oil milling process contains high organic matter from which the molecule of interest for bioconversion such as fermentable sugar can be derived. This study...

Authors: Bukhari Nurul-Adela, Abu-Bakar Nasrin and Soh-Kheang Loh

Bioformulation of Xenorhabdus stockiae PB09 for controlling mushroom mite, Luciaphorus perniciosus Rack

Bioformulations are the preparations that contain beneficial microorganisms as active ingredients and they may represent a novel alternative to be used in crop protection because of their safety to humans and ...

Authors: Piyarat Namsena, Prapassorn Bussaman and Paweena Rattanasena

Evaluation of gas supply configurations for microbial product formation involving multiple gaseous substrates

Gaseous substrates such as O₂ and CO₂ are often required in fermentation processes. However, a simple methodology to compare different gas supply strategies using gaseous substrates from different sources is miss...

Authors: Erik B. G. Häusler, Luuk A. M. van der Wielen and Adrie J. J. Straathof

Thanks to peer reviewers

Authors: Jian-He Xu

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Many methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very time-consuming. Three-phase...

Authors: Ashish G. Waghmare, Manoj K. Salve, Jean Guy LeBlanc and Shalini S. Arya

Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditions

Application of modern biomass pyrolysis methods for production of biofuels and biochar is potentially a significant approach to enable global carbon capture and sequestration. To realize this potential, it is ...

Authors: James W. Lee, Bob Hawkins, Michelle K. Kidder, Barbara R. Evans, A. C. Buchanan and Danny Day

Microwave-assisted synthesis of silver nanoparticles from Origanum majorana and Citrus sinensis leaf and their antibacterial activity: a green chemistry approach

Silver nanoparticles (SNPs) play important role in the field of optics and electronics and also as a novel antibacterial agents. Here, we report a simple and green method for the biosynthesis of SNPs using aqu...

Authors: Darshan Singh, Deepti Rawat and Isha

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradati...

Authors: Khushboo Bhange, Venkatesh Chaturvedi and Renu Bhatt

Nanosilver-decorated nanographene and their adsorption performance in waste water treatment

Water, a precious resource for all living organisms has become a diminishing source in the present world. Reuse of water is a way of combating the scarcity of water. The remedy for the polluted water through e...

Authors: M. Jannathul Firdhouse and P. Lalitha

Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

In current times, enzyme-catalyzed reactions have gained importance for the development of new chemical processes. These require the production of large quantity of enzyme at low cost. Solid-state fermentation...

Authors: Helina Patel and Akshaya Gupte

Bioremediation of Cu(II) contaminated water by Saccharum officinarum: effect of oxalic acid modification on equilibrium, kinetic and thermodynamic parameters

Copper is one of the heavy metals whose presence in aquatic environment in higher concentration poses a major threat to the environment. This is due to their toxic effects on the plants, animals and human heal...

Authors: Abideen Idowu Adeogun, Mopelola Abidemi Idowu, Kayode Olubunmi Akiode and Sikiru Akiyeye Ahmed

Asymmetric bio-epoxidation catalyzed with the styrene monooxygenase from Pseudomonas sp. LQ26

Styrene monooxygenases (SMOs) can catalyze the asymmetric epoxidation of alkenes to obtain optically active epoxides. This account describes a series of work of our group on the isolation, application, and imp...

Authors: Yan Liu, Yu-Chang Liu and Zhong-Liu Wu

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coco...

Authors: Simone Lopes do Rêgo de Oliveira, Tatiane Cavalcante Maciel, Soraya de Oliveira Sancho and Sueli Rodrigues

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO₃ using two lignin-degradi...

Authors: Anand Barapatre, Keshaw Ram Aadil and Harit Jha

Degradation and detoxification of synthetic dyes and textile industry effluents by newly isolated Leptosphaerulina sp. from Colombia

Wastewaters from the textile industry are an environmental problem for the well-known Colombian textile industry. Ligninolytic fungi and their enzymes are an option for the treatment of these wastewaters; howe...

Authors: Jersson Plácido, Xiomara Chanagá, Santiago Ortiz-Monsalve, María Yepes and Amanda Mora

Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

Optimization of chemically defined medium has been a critical way to produce monoclonal antibody. Usually, amount of glutamine was added into the feed medium, but a half of asparagine was added. Our study foun...

Authors: Li-xiang Zhang, Wei-yan Zhang, Chen Wang, Jin-tao Liu, Xian-cun Deng, Xu-ping Liu, Li Fan and Wen-song Tan

Development and evaluation of a functional bioreactor for CO fermentation into ethanol

In a conventional syngas fermentation process, gas was released into the fermentation broth through a single orifice or multiple orifices, except the hollow fiber membrane reactor. Consequently, a simplified b...

Authors: Poritosh Roy, Animesh Dutta and Sheng Chang

Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease

Cross-linked enzyme aggregate (CLEA) is considered as an effective technique in the production of immobilized biocatalysts for its industrially attractive advantages. Simplicity, stability, low cost, time savi...

Authors: Safa Senan Mahmod, Faridah Yusof, Mohamed Saedi Jami and Soofia Khanahmadi

Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity

As nonionic surfactants derived from naturally renewable resources, sugar fatty acid esters (SFAEs) have been widely utilized in food, cosmetic, and pharmaceutical industries.

Authors: Xiao-Sheng Lin, Kai-Hua Zhao, Qiu-Ling Zhou, Kai-Qi Xie, Peter J. Halling and Zhen Yang

Bioprocess modelling for the design and optimization of lignocellulosic biomass fermentation

Lignocellulosic feedstocks, which are currently under-exploited, can be used for the production of biofuels, such as ethanol, and for biorefinery applications to produce a variety of value-added products. Alth...

Authors: Pornkamol Unrean

Current applications of different type of aqueous two-phase systems

In recent year, aqueous two-phase system (ATPS) has become a proven tool used in separation and purification technology. The application of ATPSs in clarification, partitioning and partial purification of biom...

Authors: Yee Koon Yau, Chien Wei Ooi, Eng-Poh Ng, John Chi-Wei Lan, Tau Chuan Ling and Pau Loke Show

A statistical approach on optimization of exopolymeric substance production by Halomonas sp. S19 and its emulsification activity

An exopolymer producing bacterial strain was identified as Halomonas sp. S19 by 16S rRNA gene sequencing isolated from Mandapam, Southeast coast of India. Strain S19 produces a significant amount of exopolymer (...

Authors: Parthiban Karuppiah, Vignesh Venkatasamy, Nilmini Viswaprakash and Thirumurugan Ramasamy

Biosynthesis of nanoparticles and silver nanoparticles

In this century, the development of nanotechnology is projected to be the establishment of a technological evolutionary of this modern era. Recently, nanotechnology is one of the most active subjects of substa...

Authors: Cheah Liang Keat, Azila Aziz, Ahmad M Eid and Nagib A. Elmarzugi