Articles

Microbial degradation of petrochemical waste-polycyclic aromatic hydrocarbons

Petrochemical industry is one of the fastest growing industries. This industry has immense importance in the growth of economy and manufacture of large varieties of chemicals. The petrochemical industry is a h...

Authors: M. H. Fulekar

Lobster processing by-products as valuable bioresource of marine functional ingredients, nutraceuticals, and pharmaceuticals

The worldwide annual production of lobster was 165,367 tons valued over $3.32 billion in 2004, but this figure rose up to 304,000 tons in 2012. Over half the volume of the worldwide lobster production has been...

Authors: Trung T. Nguyen, Andrew R. Barber, Kendall Corbin and Wei Zhang

Detoxification of parthenium (Parthenium hysterophorus) and its metamorphosis into an organic fertilizer and biopesticide

Vermicompost of the toxic and allelopathic weed parthenium (Parthenium hysterophorus) was explored for its possible use as an organic fertilizer. Replicated plant growth trials were conducted using four levels of...

Authors: Naseer Hussain, Tasneem Abbasi and Shahid Abbas Abbasi

Beechwood carbohydrates for enzymatic synthesis of sustainable glycolipids

Moving away from crude oil to renewable resources for the production of a wide range of compounds is a challenge for future generations. To overcome this, the use of lignocellulose as substrate can contribute ...

Authors: Sascha Siebenhaller, Tatjana Hajek, Claudia Muhle-Goll, Miriam Himmelsbach, Burkhard Luy, Frank Kirschhöfer, Gerald Brenner-Weiß, Thomas Hahn, Susanne Zibek and Christoph Syldatk

Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study.

Authors: Xiaoling Wu, Cheng Yang and Jun Ge

Mineralization of a sulfonated textile dye Reactive Red 31 from simulated wastewater using pellets of Aspergillus bombycis

Reactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis st...

Authors: Razia Khan and M. H. Fulekar

Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs

Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial ...

Authors: Rui Ye, Mingzhi Huang, Hongzhong Lu, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang and Siliang Zhang

Enhancement of asymmetric bioreduction of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine to corresponding (S)-enantiomer by fusion of carbonyl reductase and glucose dehydrogenase

(S)-(−)-N,N-Dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine (DHTP) is a key intermediate for the preparation of (S)-duloxetine, an important antidepressant drug. However, so far, the catalytic efficiency of (S)-DH...

Authors: Taiqiang Sun, Bin Li, Yao Nie, Dong Wang and Yan Xu

Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

Copper oxidase is a promising enzyme for detection of oxidation, which can function as a biosensor and in bioremediation. Previous reports have revealed that the activity of the multicopper oxidase (MCO, EC 1....

Authors: Shih-I Tan, I-Son Ng and You-Jin Yu

Utilization of corncob xylan as a sole carbon source for the biosynthesis of endo-1,4-β xylanase from Aspergillus niger KIBGE-IB36

Xylan is a hemicellulose polysaccharide which is composed of β-1,4-linked d-xylosyl residues. Endo-1,4-β xylanase has the ability to cleave xylan back bone chains to release xylose residues. They are produced by ...

Authors: Urooj Javed, Afsheen Aman and Shah Ali Ul Qader

Food waste: a potential bioresource for extraction of nutraceuticals and bioactive compounds

Food waste, a by-product of various industrial, agricultural, household and other food sector activities, is rising continuously due to increase in such activities. Various studies have indicated that differen...

Authors: Krishan Kumar, Ajar Nath Yadav, Vinod Kumar, Pritesh Vyas and Harcharan Singh Dhaliwal

Industrial cellulase performance in the simultaneous saccharification and co-fermentation (SSCF) of corn stover for high-titer ethanol production

Cellulase enzymes contribute to the largest portion of operation cost on production of cellulosic ethanol. The industrial cellulases available on the industrial enzyme market from different makers and sources ...

Authors: Qiang Zhang and Jie Bao

Lignocellulases: a review of emerging and developing enzymes, systems, and practices

The highly acclaimed prospect of renewable lignocellulosic biocommodities as obvious replacement of their fossil-based counterparts is burgeoning within the last few years. However, the use of the abundant lig...

Authors: Eugene M. Obeng, Siti Nurul Nadzirah Adam, Cahyo Budiman, Clarence M. Ongkudon, Ruth Maas and Joachim Jose

Review on the current status of polymer degradation: a microbial approach

Inertness and the indiscriminate use of synthetic polymers leading to increased land and water pollution are of great concern. Plastic is the most useful synthetic polymer, employed in wide range of applicatio...

Authors: Vinay Mohan Pathak and Navneet

Biofibres from biofuel industrial byproduct—Pongamia pinnata seed hull

Biodiesel production using Pongamia pinnata (P. pinnata) seeds results in large amount of unused seed hull. These seed hulls serve as a potential source for cellulose fibres which can be exploited as reinforceme...

Authors: Puttaswamy Manjula, Govindan Srinikethan and K. Vidya Shetty

Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations

Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (rang...

Authors: Zhimeng Wu, Haofei Hong, Xinrui Zhao and Xun Wang

Techno-economic analysis of extraction-based separation systems for acetone, butanol, and ethanol recovery and purification

Dual extraction, high-temperature extraction, mixture extraction, and oleyl alcohol extraction have been proposed in the literature for acetone, butanol, and ethanol (ABE) production. However, energy and econo...

Authors: Víctor Hugo Grisales Díaz and Gerard Olivar Tost

Advances in industrial microbiome based on microbial consortium for biorefinery

One of the important targets of industrial biotechnology is using cheap biomass resources. The traditional strategy is microbial fermentations with single strain. However, cheap biomass normally contains so co...

Authors: Li-Li Jiang, Jin-Jie Zhou, Chun-Shan Quan and Zhi-Long Xiu

Changes of membrane fatty acids and proteins of Shewanella putrefaciens treated with cinnamon oil and gamma irradiation

In order to detect the antimicrobial mechanism of combined treatment of cinnamon oil and gamma irradiation (GI), the membrane fatty acids and proteins characteristics of Shewanella putrefaciens (S. putrefaciens) ...

Authors: Fei Lyu, Fei Gao, Qianqian Wei and Lin Liu

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expres...

Authors: Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun and Dawei Zhang

An efficient multi-stage fermentation strategy for the production of microbial oil rich in arachidonic acid in Mortierella alpina

Fungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage ...

Authors: Wen-Jia Wu, Ai-Hui Zhang, Chao Peng, Lu-Jing Ren, Ping Song, Ya-Dong Yu, He Huang and Xiao-Jun Ji

Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review

Lignocellulosic feedstock materials are the most abundant renewable bioresource material available on earth. It is primarily composed of cellulose, hemicellulose, and lignin, which are strongly associated with...

Authors: Adepu Kiran Kumar and Shaishav Sharma

Fungal pretreatment of raw digested piggery wastewater enhancing the survival of algae as biofuel feedstock

Understanding about the impact of white rot fungi on indigenous bacterial communities, NH₄ ⁺ and turbidity in digested piggery wastewater, will allow the optimization of wastewater trea...

Authors: Junying Liu, Wen Qiu and Yunpu Wang

Hydrolysis of carotenoid esters from Tagetes erecta by the action of lipases from Yarrowia lipolytica

The present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from Tagetes erecta using lipases from the yeast of Yarrowia lipolytica, with the aim of obtaining free lut...

Authors: Abraham Figueiras Abdala, Alfonso Pérez Gallardo, Lorenzo Guevara Olvera and Eleazar Máximo Escamilla Silva

Investigation of spiral-wound membrane modules for the cross-flow nanofiltration of fermentation broth obtained from a pilot plant fermentation reactor for the continuous production of lactic acid

The separation performance of seven polymer membranes for the nanofiltration of sodium lactate in fermentation broth was investigated. Each module was introduced into the test stand, and the system curve was o...

Authors: Hendrik Laube, Roland Schneider and Joachim Venus

Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtain...

Authors: Doria Naila Bouchedja, Sabine Danthine, Tambi Kar, Patrick Fickers, Abdelghani Boudjellal and Frank Delvigne

Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase

(S)-Mandelate dehydrogenase (SMDH) and laccase were immobilized on chitosan. The bi-enzymatic system with immobilized SMDH and immobilized laccase was taken to catalyze the stereoselective transformation of ra...

Authors: Xing Chen, Chengli Yang, Peng Wang, Xuan Zhang, Bingxin Bao, Dali Li and Ruofu Shi

Dissolved oxygen control strategy for improvement of TL1-1 production in submerged fermentation by Daldinia eschscholzii

2,3-Dihydro-5-hydroxy-2-methylchromen-4-one (TL1-1) is a phenolic compound with significant anti-fungal and anti-cancer activities produced by Daldinia eschscholzii (D. eschscholzii). However, studies have rarely...

Authors: Xing-chen Wei, Liu Tang and Yan-hua Lu

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

This study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.

Authors: Dharmender Kumar, Abhijit Kumar and Jyoti Sharma

Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin

Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites.

Authors: Xin Lv, Lu Dai, Fangmin Bai, Zhanqing Wang, Liaoyuan Zhang and Yaling Shen

Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production

The cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing the strain’s...

Authors: Hongxing Li, Yu Shen, Meiling Wu, Jin Hou, Chunlei Jiao, Zailu Li, Xinli Liu and Xiaoming Bao

Electricity generation from rice bran in microbial fuel cells

Rice bran is a by-product of the rice milling process and mostly discarded in Japan. Although many studies have shown that microbial fuel cells (MFCs) are able to generate electricity from organic wastes, limi...

Authors: Shu Takahashi, Morio Miyahara, Atsushi Kouzuma and Kazuya Watanabe

Penicillin acylase-catalyzed synthesis of N-bromoacetyl-7-aminocephalosporanic acid, the key intermediate for the production of cefathiamidine

Enzymatic approaches have become promising alternatives to chemical methods for the production of semi-synthetic β-lactam antibiotics. In this work, enzymatic synthesis of N-bromoacetyl-7-aminocephalosporanic aci...

Authors: Xiao-Li Zhang, Min-Hua Zong and Ning Li

In silico profiling of cell growth and succinate production in Escherichia coli NZN111

Succinic acid is a valuable product due to its wide-ranging utilities. To improve succinate production and reduce by-products formation, Escherichia coli NZN111 was constructed by insertional inactivation of lact...

Authors: Xingxing Jian, Ningchuan Li, Cheng Zhang and Qiang Hua

Combined ¹³C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, en...

Authors: Ping Liu, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang and Siliang Zhang

Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

Streptomyces aureofaciens strain A6-9, obtained with traditional mutagenesis, produces elevated levels of 6-DCT. The increased formation of 6-DCT may be attributable to the changes in ...

Authors: Hong Li, Ruifang Ye, Guizhen Lin, Deyu Zhu and QuanGui Mao

Improvement of chaetominine production by tryptophan feeding and medium optimization in submerged fermentation of Aspergillus fumigatus CY018

Chaetominine (CHA) is a novel alkaloid with excellent medicinal activities produced by Aspergillus fumigatus CY018. However, its further application has been severely restricted by the low production yield. In th...

Authors: Yu-peng Zhang, Rui-hua Jiao, Yan-hua Lu and Ling-yun Yao

Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the gener...

Authors: Zhoutong Sun, Pamela Torres Salas, Elina Siirola, Richard Lonsdale and Manfred T. Reetz

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

The organic nitrogen source is one of the key factors affecting Mortierella alpina cell growth as well as arachidonic acid (ARA) production. The aim of the present work is to achieve an optimized recipe of organi...

Authors: Xue-ping Ling, Si-yu Zeng, Cui-xue Chen, Xiao-ting Liu and Ying-hua Lu

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural c...

Authors: Pallavi Singh and Bhupendra N. Tiwary

Preliminary integrated economic and environmental analysis of polyhydroxyalkanoates (PHAs) biosynthesis

Being biodegradable and renewable, polyhydroxyalkanoates (PHAs), a green polymer, attract much attentions as potential alternative for conventional plastics due to increased concern towards environmental issue...

Authors: Yoong Kit Leong, Pau Loke Show, Heng Chou Lin, Chih Kai Chang, Hwei-San Loh, John Chi-Wei Lan and Tau Chuan Ling

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness. Hydrocarbon-polluted soils h...

Authors: Ijeoma Vivian Nwaguma, Chioma Blaise Chikere and Gideon Chijioke Okpokwasili

Effects of hot-washing process on structure and enzymatic hydrolysis of treated steam explosion corn stover

Cellulase adsorption of lignocellulosic materials is the key link during enzymatic hydrolysis. Hot-washing process (above lignin glass transition temperature) was used to change the physical structure of ligni...

Authors: Xu Yang, Xiaojian Ma, Hongliang Li, Junyin Chen and Shuqi Fang

Microbial fuel cell: a green approach for the utilization of waste for the generation of bioelectricity

Today we are witnessing a global energy crisis due to huge energy demands and limited resources. Non-renewable energy sources are depleting and renewable energy sources are not properly utilized. There is an i...

Authors: Venkatesh Chaturvedi and Pradeep Verma

Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system

Porcine circovirus type 2 (PCV2) virus-like particles (VLPs) are an effective vaccine against post-weaning multisystemic wasting syndrome. Burst sequence (BS) and the chicken 5′-HS4 β-globin (HS4) insulator we...

Authors: Yingjun Li, Xiaoping Yi, Yingping Zhuang and Ju Chu

Erratum to: Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditions

Authors: James W. Lee, Bob Hawkins, Michelle K. Kidder, Barbara R. Evans, Sokwon Paik, A. C. Buchanan and Danny Day

Cultivation of Nannochloropsis sp. using narrow beam angle light emitting diode in an internally illuminated photobioreactor

This paper reports on the growth condition of Nannochloropsis sp. in an annular column-type photobioreactor (PBR) using light-emitting diode as an internal illumination.

Authors: Mohamad Taisir, Chee Loong Teo, Ani Idris and Affendi M. Yusuf

Investigating the mechanism of nanofiltration separation of glucosamine hydrochloride and N-acetyl glucosamine

Glucosamine hydrochloride (GAH) and N-acetyl glucosamine (NAG) are chitin derivatives. Owing to their excellent biological activity, they have long been used as pharmaceuticals and nutraceuticals. However, both o...

Authors: Shengnan Zhang, Jiachun Zhou, Liqiang Fan, Yongjun Qiu, Lihua Jiang and Liming Zhao

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

This study investigated the effect of aeration rate and light intensity on biomass production and total fatty acids (TFA) accumulation by Porphyridium purpureum. The red microalgae is also known to accumulate con...

Authors: Gaomin Su, Kailin Jiao, Jingyu Chang, Zheng Li, Xiaoyi Guo, Yong Sun, Xianhai Zeng, Yinghua Lu and Lu Lin

Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa

The FadR subfamily of regulators plays essential roles in the regulation of diverse metabolic pathways in bacteria. LldR, an FadR-type regulator, regulates lactate utilization in Pseudomonas aeruginosa.

Authors: Bo Xin, Geng Wu, Kunzhi Zhang, Yongxing He, Hongzhi Tang, Chao Gao, Ping Xu and Cuiqing Ma