In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2

The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography–mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future.


Introduction
Fungi are a major source of metabolites with a wide range of biological and therapeutic activities (Baker and Alvi 2004). The fungal secondary metabolites have been reported as antibiotics, therapeutic agents as well as undesirable immunosuppressant and toxic substances (Miranda et al. 2010). They also show antimicrobial (erythromycin and bacitracin), antiproliferative, antiaging, anti-inflammatory, anticancer (Maheshwari et al. 2017), hypocholesterolemic (Kwon et al. 2002), antifungal (Nikoletti et al. 2007) antiviral (Nishihara et al. 2000) and antioxidant (Gangadevi and Muthumary 2008) activities. The natural antioxidant activity of fungal secondary metabolites thus has a plausible major role in developing therapeutic interventions against cancer and myocardial infarction (Maritim et al. 2003).
The fungal metabolites have been also reported to regulate different metabolic pathways and cellular activities due to pleiotropic action (Badri et al. 2009). Rhizospheric fungi are a major untapped source of novel metabolites Sahu et al. Bioresour. Bioprocess. (2020) 7:14 which have not yet been explored and screened to assess their therapeutic potential. Thus, in the present study, fungi isolated from pristine soil of the Achanakmar Biosphere region (located in central India) were evaluated for their potent biological activities, extraction, and identification of novel secondary metabolites with putative therapeutic potentials using standard biochemical and cell biological approaches. Briefly, the extract of the strain with potent activity was selected and the secondary metabolites were purified by adsorption column chromatography, gas chromatography-mass spectroscopy (GC-MS) and nuclear magnetic resonance (NMR) techniques which were subsequently evaluated for antioxidant, antimicrobial and antiproliferative activities using in vitro analysis that corroborated well with in silico molecular docking analysis.

Isolation and identification of the fungus
Different fungal strains were isolated from the rhizospheric soil of Achanakmar Biosphere, Bilaspur, Chhattisgarh, India, by the method as described earlier (Radhika and Rodrigues 2010). The potent fungal isolate was characterized and identified based on morphological characterization using a compound microscope (AXIO SCOPE. A1 HBO 50, Zeiss, Germany) and scanning electron microscope (FEI Nova NanoSEM450, Thermo Fisher USA). Molecular characterization of the identified fungal isolate was performed by partial gene sequencing of the internal transcribed spacer (ITS) regions at a commercial center (Chromous Biotech Pvt. Ltd., Bangalore, India). Briefly, fungal DNA was isolated using a DNA isolation kit (Invitrogen, USA). PCR was performed for amplification of the ITS region with fungal ITS specific degenerate primer (forward primer 5′-TCMGTA GGT GADCCWBCGS-3′ and reverse primer 5′-TCCTNC-GYTKATKGVTADGH-3′) followed by amplified ITS sequence alignment with similar sequences of other fungi using the BLASTN program (NCBI, USA). Mega 6 software (Pennsylvania State University, USA) was used for the construction of the phylogenetic tree with 26 aligned sequences of fungi using maximum likelihood analysis and Tamura 3-parameter nucleotide substitution methods (Aharwar and Parihar 2019).

Purification of ethanolic extract
Ethanolic extract was purified to obtain active metabolites for further investigation. In brief, 1 g of dry extract was mixed with ethanol (1:1 w/v) and subjected to adsorption chromatography on a glass column packed with silica gel (60-120 mesh size) in toluene. Elution was carried out by standard method with increasing polarity of toluene, chloroform, ethyl acetate, methanol, and acetonitrile. Fractions obtained from each solvent were collected and subjected to spectrophotometric evaluation and selected fractions were further concentrated by the rotatory evaporator.

High-performance liquid chromatography (HPLC) analysis
The collected extracts and fractions were used for initial screening on TLC plates. The screening sample was dissolved in (1:1 w/v) in HPLC grade methanol. The purity of the compound was confirmed by HPLC (Shimadzu Liquid Chromatography LC10A; Shimadzu Corp., Kyoto, Japan) using a C 18 analytical column. Injected sample (20 µL) was eluted at a flow rate of 1 mL/min under an isocratic mobile phase consisting of acetonitrile: water: acetic acid (18:80:2) (v/v) followed by analysis of elution profile at 280 nm (Shen et al. 2007).

Identification of compound using spectroscopy techniques UV-visible spectroscopy
The sample was dissolved in ethanol. The absorption maximum (λmax) of the purified compound was determined (UV-1800 Shimadzu spectrophotometer, Shimadzu, Kyoto, Japan) by scanning over 200-800 nm range to record the spectrum. 1 H NMR of the sample was performed on Bruker advance III 400 MHz NMR spectrophotometer. In brief, 5 mg of the sample was dissolved in the DMSO and centrifuged at 8000 rpm for 10 min and then analyzed for 1 H NMR as described earlier (Morcombe and Zilm 2003). Chemical shifts were expressed in terms of parts per million (į scale) and elemental analysis was carried out at Centre for Bio-separation Technology (CBST), Vellore Institute of Technology, Vellore, India. The data were used for the putative prediction of molecular formula and structural characteristics of the active compound comparing with the standards.

Bioactive properties of isolated compound
The antioxidant and antimicrobial functional characteristics of the isolated pure compound from T. purpureogenus and extracts were evaluated in triplicates with appropriate blanks and controls (ascorbic acid for antioxidant and streptomycin for antibacterial activity) in all the experiments.

2,2-Diphenyl 1-picrylhydrazyl (DPPH) activity radical scavenging activity
The DPPH (0.2 mM) radical scavenging activity was measured using modified method as described previously (Dhale and Vijay-Raj 2009). The activity was evaluated using various concentrations (31.25-125 µg/mL) of purified compound at 517 nm and calculated according to the formula: where [A 0 ] was the absorbance of the control (DPPH without sample) and [A 30 ] was the absorbance of the sample with DPPH ( Barapatre et al. 2015).

Ferric reducing antioxidant power (FRAP) assay
The ferric reducing antioxidant power (FRAP) assay was determined, according to the method of Benzie and Strain (1996). Briefly, when a ferric tripyridyltriazine (Fe III-TPTZ) complex gets reduced to the ferrous (Fe II) form at low pH, an intense blue color appears. Low pH is responsible for maintaining iron solubility and a decrease in the ionization potential that drives electron transfer and increases the redox potential. The FRAP activity of the sample was determined at various concentrations (66.7-166.7 µg/mL) by observing an increase in the absorbance values at 595 nm.

Phosphomolybdenum assay
Phosphomolybdenum assay method was utilized for the spectrophotometric quantitation of total antioxidant capacity by combining the sample with 1 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate) followed by incubation at 95 °C for 90 min. The samples were cooled to room temperature, and the absorbance of the test solution was measured at 695 nm against a blank (Sowndhararajan and Kang 2013).

Antimicrobial activity
The antibacterial activity assay was carried out using disc diffusion method (Balachandran et al. 2016 inhibition. Ethanol was used as vehicle control and streptomycin (1 mg/mL) as positive control.

In silico analysis by molecular docking
Molecular docking of isolated and characterized fungal metabolite, 3-methyl-4-oxo-pentanoic acid with standard anticancer and anti-aging drugs was carried out against selected target proteins to study the binding-affinity,energy, -mode and scoring functions. The structure of the targets was retrieved from the Protein Data Bank (PDB). Molecular docking and ligand-receptor interactions study was carried out using Molecular Operating Environment 2008 (moe.2008) software (Chemical Computing Group, Montreal, Canada) to investigate possible binding conformations of the receptor-ligand complex (Naik et al. 2011). For anti-aging potential the targets chosen were yeast Taf14 containing YEAST domain at N-terminus (Schulze et al. 2010 (Misra et al. 2000) was chosen for docking study.

Anti-aging analysis
Traditional spot assay was employed for evaluation of the anti-aging capacity of identified compounds in eukaryotic haploid organism (Yeast) Saccharomyces cerevisiae specific mutant strain BY4742 (MTCC-3157) as described earlier . BY4742 strain of S. cerevisiae was inoculated in 5 mL YPD (Yeast Peptone Dextrose broth (Hi-Media, Mumbai, MH, India) and incubated overnight at 28 ± 2 °C till exponential phase. 40 µL fungal extract was spotted in the YPD agar plate, fluconazole (Nystatin) was used as negative control while culture without extract, acarbose, and rapamycin were treated as positive control. Growth of yeast strain was observed after incubation of 72 h at 28 ± 2 °C. Further yeast growth curve determination was also performed using the method of Wei et al. (2017): Delaney et al. (2013): Tauk tornisielo et al. (2007 with slight modification. S. cerevisiae BY 4742 inoculum was prepared using yeast dextrose peptone nutrient medium at 28 °C. 100 µL of extract (10 mg) was taken and 100 µL of inoculum was added. Acarbose and rapamycin were taken as control. Absorbance was taken at 600 nm using ELISA Reader at different time points.

Antiproliferative activity
The antiproliferative activity of the extracts and fractions were determined in various tissue-specific cancer cells lines such as breast cancer (MDA-MB-231, MDA-MB-468, and MCF-7), liver cancer (HepG2), lung cancer (A-549), prostate cancer (DU-145) and primary control cell line (HEK-293) by sulforhodamine B (SRB) assay as described previously (Manupati et al. 2017). Briefly, each cell line was trypsinized and plated in 96-well plate at density 5 × 10 3 cells per well. After 24 h incubation, cells were treated with increasing concentration (1, 10, 100 and 300 mg/mL) of all the column fractions viz. toluene, chloroform, ethyl acetate, methanol, and acetonitrile for 48 h followed by fixation and SRB staining of treated cells along with respective vehicle controls. Doxorubicin was used as a positive control. The optical density at 510 nm was measured using a multimode reader (Perkin Elmer, Germany) and percent inhibition with IC-50 was calculated using GraphPad prism 6.0 as described previously (Manupati et al. 2019).

Statistical analysis
All assays were performed in triplicates and the results were validated statistically using one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test. All the tests were considered statistically significant at p < 0.05. The analysis was carried out using GraphPad Prism Software Version 5.0. Results were represented as the mean ± standard deviation (SD).

Results
In the present work, isolated fungal strain, Talaromyces purpureogenus isolate-ABRF2 from the soil sample of Achanakmar Biosphere Reserve of Chhattisgarh forest, India, was screened for potential therapeutic secondary metabolites. Initially, we cultured the fungus in different nutrient media to identify the optimum growth conditions. The ethanolic extract of the isolated fungus Talaromyces purpureogenus isolate-ABRF2 was subjected to preliminary screening based on antioxidant and antibacterial activity. Potent crude extract (brown sticky, Fig.  S1A) was further column purified based on the polarity of the solvent. Purified bioactive secondary metabolites were identified using analytical techniques such as UV-visible spectrophotometry, TLC, HPLC, GC-MS, and NMR. The identified bioactive metabolite, 3-methyl-4-oxo-pentanoic acid (Additional file 1: Fig. S1B) was evaluated for antimicrobial, anticancer and anti-aging properties through in vitro and in silico studies.

Molecular taxonomic characterization and phylogenetic analysis of fungal isolate
The isolate, ABRF2 was morphologically characterized by cotton blue staining (Additional file 1: Fig. S2A, S2B), bright field microscopy (Additional file 1: Fig. S2C) and scanning electron microscopy (Additional file 1: Fig. S2D). It displayed white green color mycelia, with ellipsoidal conidia, thick-walled and dark red coloration, dense sporulation and plane surface colony. Colonies produce red soluble pigments on nutrient media. Molecular characterization of fungi was performed using the PCR technique, agarose gel electrophoresis and sequencing reaction with the ITS rDNA sequence. Agarose gel electrophoresis was used for the analysis of isolated genomic DNA and the PCR amplified product of the ITS region of the fungal isolate. The amplicon size of the PCR was observed to be 422 base pairs. The amplicon was sequenced and submitted to the GenBank database (NCBI accession number, MG905442). It was further analyzed for sequence similarity using the BLASTN program of NCBI. The sequence showed a maximum of 72% similarity with Talaromyces purpureogenus strain NFML_X. A phylogenetic tree was prepared by 'maximum likelihood'-a statistical method using Mega 6 software with the Tamura 3-parameter model (Substitution Model) and bootstrap method (phylogeny test). The phylogenetic tree of selected taxa was formed by the neighbor-joining method. The model used was Tamura 3-parameter with 1000 Boost strap replications. The total numbers of sites were 3555.

Structural characterization by spectroscopic methods
The potent crude ethanolic extract and column purified fraction of isolate ABRF2 was further spotted on a silica gel TLC sheet. The components of the sample were separated by TLC and observed by visualization at UV-366 nm. Crude ethanolic extract (spot A and B) and column fractions of methanolic extract, on TLC separation, resolved to give fluorescent spots under UV light (spot D). The R f value for spot A was observed to be 0.15 while that for spot B and C was found to be 0.36. The R f values of spot D was observed to be 0.41 (Additional file 1: Fig. S3). The HPLC analysis of Talaromyces purpureogenus isolate-ABRF2 fungus with gradient elution depicted nine peaks in its ethanol extract spectra (Fig. 1a). The peaks with the following retention time (min) 2. 35, 2.55, 2.8167, 3.4167, 3.8, 3.9167, 4.6, 4.85 and 7.0167 were resolved. The compound of interest was eluted at Rt 2.8167. It was soluble in organic solvents including methanol, chloroform, acetone and ethanol (Fig. 1a). The structure of the selected compound was analyzed using UV-visible spectroscopy. Ethanolic fraction of fungal extract depicted absorption spectra overlapping with that of standard pentanoic acid, suggesting the isolated compound to be pentanoic acid (C 5 H 10 O 2 ) with absorption spectra between 300 and 400 nm (Fig. 1b). The purified compound when scanned over a range of 200-800 nm, exhibited peaks between 300 and 400 nm, suggesting the compound to be benzoic acid derivative (Fig. 1b). GC-MS analysis of the ethanolic fungal extract and purified fraction showed the presence of six major peaks (Fig. 1c).
The components corresponding to the peaks were determined and represented in tabular form (Additional file 1: Table S1). Pentanoic acid, 3-methyl-4-oxo-was one of the metabolites (molecular weight, 103 g/mol) selected to determine its biological significance while the remaining of the detected peaks/compounds corresponded to the solvents like methyl alcohol, cyclopropane, and propane nitrile with relatively out of scope for therapeutic relevance in the present study.
The column-fractionated samples were subjected to the FTIR analysis for structural elucidation of the compound. A broad range of bands (3910-3660 cm −1 ) were observed in the chloroform and ethyl acetate fraction that can be attributed to the -OH group stretching in phenolic and aliphatic structures and oscillation of the hydroxyl group. A characteristic peak that appeared at 3155 cm −1 represented the stretching of -OH group, molecular hydrogen bonding and vibration of molecules. The IR spectrum depicted the presence of an aromatic ring substituted with an ester bond thereby predicting the probable functional group present in the compound. Putative prediction of the isolated compound identified it to be a pyrone derivative (Fig. 2 and Additional file 1: Table S2). The NMR spectrum of column fraction of the compound was further evaluated for structural identification using the obtained peaks at various 'į' values of proton 1 H (Fig. 3). The data obtained has led us to the presumption that the antibacterial compound contains methyl, ketone, and hydroxyl functionalities. The NMR spectrum showed that the antibacterial compound has CH2, CH3, OH, and C-H protons functionalities while the elemental data showed the presence of carbon, hydrogen and hydroxyl group suggesting the compound to be an ester. The 1 H NMR spectrum of the isolated compound, 3-methyl-4-oxo-pentanoic acid corroborated well with the same number of protons as pentanoic acid (Fig. 3). A comparative analysis of the 1 H NMR spectrum has been described in tabular form (Additional file 1: Table S3). This shows that the primary, secondary and tertiary aliphatic group presence of a broad peak at 0.9, 1.3 and 1.5 δ, respectively. The carbonyl and hydroxyl groups were intact at 2.2 and 3.5 δ, respectively, although some changes in chemical shifts were noticed.

Antioxidant activity
Antioxidants provide cellular defense through entrapping free radical generated by toxic metals and series of mechanisms get initiated by terminating chain reaction or chelating metal ions and reactive oxygen species or by maintaining the redox potential to stop or minimize reduction of molecular oxygen. Our findings indicate the presence of a higher concentration of potential reductones, 83% scavenging activity in YESB crude extract as compared with other extracts (Fig. 4). Hence, the FRAP reaction was correlated with the varying concentrations of the antioxidants that were observed to be reproducible (Fig. 4). The DPPH radical scavenging activity of secondary metabolites in the different medium was observed to be in the range of 18-79%. However, crude extract in YESB media demonstrated the highest DPPH radical scavenging activity at 79%. The activity of the crude extract was comparable to that of control, ascorbic acid. Ascorbic acid and un-inoculated media were used as positive and negative controls, respectively. Free radicals often affect the cellular macromolecules and signaling mechanisms while antioxidants protect it. We observed that YESB extract, with 80% scavenging capacity was very strong in the cation-free radical quenching compared to other extracts, thereby suggesting the presence of relative hydrophobic reductones in the latter. Formation of a bluish-green colored complex at acidic pH signifies the reduction of phosphomolybdate (VI) to phosphomolybdate (V) during the phosphomolybdenum assay. The highest percentage of scavenging activity of the crude extract was observed in the YESB medium (74%), although a significant difference in scavenging activity was observed between the positive control, ascorbic acid, and crude extract (Fig. 4). Thus, these data suggest YESB extract has potent free-radical scavenging activity.
Antifungal activity of the crude fungal extract and column-fractionated sample obtained were evaluated for antifungal activity against C. albicans (MTCC-3017) (Additional file 1: Table S5). Plate depicted the zone of inhibition (10.11 ± 0.89) of acetonitrile column fraction of ethanolic extract comparable to the standard antifungal drug, fluconazole tested against C albicans (MTCC-3017) (Fig. 5).

Anti-aging activity Prediction of anti-aging potential by molecular docking
Multi-functional fungal extract regulating central cellular metabolism and metabolic pathways may serve as an effective anti-aging entrant. Therefore, molecular docking analysis was undertaken as described in the methods to dock 3-methyl-4-oxo-pentanoic acid against the chosen yeast and human anti-aging targets. The fungal metabolite, 3-methyl-4-oxo-pentanoic acid suggested the plausible role to support the growth of eukaryotic cells as demonstrated in the yeast. Slow aging may also lead to delay in carcinogenesis; reports suggest that calorie restriction affects aging by neutralizing . Interestingly, our observations from the in silico analysis followed by in vitro validation assays using standard drug, sirolimus (rapamycin) suggested that 3-methyl-4-oxo-pentanoic acid has a potent anti-aging property (Tables 1 and 2). The binding energy of 3-methyl-4-oxo-pentanoic acid for site 1 of the target 1AH8 target, obtained from MDA-MB-231 cell line, was observed to be − 14.0109 kCal/mol which was lower than standard drug, Sirolimus (rapamycin, − 12.7754 kCal/mol), thereby suggesting a better interaction and stability of 3-methyl-4-oxo-pentanoic acid as compared with sirolimus. The presence of seven hydrogen bonds reacting with residues Lys A73, Val A74, Arg A75, Ile A54, Phe A63, Gln A206, and Glu A76 was observed. Val A74 and Val A208 were also involved in interaction through the water of hydration (Fig. 6a).
The binding energy of 3-methyl-4-oxo-pentanoic acid was observed to be -9.9277 kCal/mol for site 8 of the target, 2KM1 or Yeast protein Dre2 containing Fe-S with S-adenosyl methionine methyltransferase-like domain (RCSB PDB ID: 2KM1). However, for sirolimus (rapamycin) the binding energy was observed to be − 13.4799 kCal/mol, lower than the isolated compound. Only two residues showed direct binding Leu 63 and Phe 73 (Fig. 6b).
In yeast, Taf14 containing the YEATS domain at N-terminus (RCSB PDB ID: 2L7E) is involved in transcription (Shanle et al. 2015). The binding energy of 3-methyl-4-oxo-pentanoic acid was observed to be − 9.6630 kCal/ mol for site 2 of the target 2L7E which was comparable to that of sirolimus (− 9.5644 kCal/mol). Four hydrogen bonds were formed between residues Leu 86, Pro 102, Leu 108, and Gly83 (Fig. 6c). This binding analysis with  Ascorbic acid was used as positive control. One-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test. Sample with p < 0.05 were considered significant p < 0.05 and ≠ p < 0.01 Sahu et al. Bioresour. Bioprocess. (2020) 7:14 different amino acids of the target sites suggested that the compound 3-methyl-4-oxo-pentanoic acid to be a potential regulator of different anti-aging pathways.

Spot assay and yeast growth curve
To validate the anti-aging activity, spot assay was performed with Saccharomyces cerevisiae specific mutant   . Negative control, nystatin and positive control, acarbose and/or Table 2 Table representing   rapamycin (1 mg/mL) were used in the experiment. The fungal extract showed a spot diameter of 12 ± 0.46 mm while the positive controls, acarbose, and rapamycin depicted 15 ± 0.87 and 17 ± 0.94 mm, respectively, in the study using S. cerevisiae BY4742 (Fig. 7a and Additional file 1: Table S6). The growth signifies the putative antiaging effect of 3-methyl-4-oxo-pentanoic acid through the calorie restriction pathway. Furthermore, yeast growth curve depicted an increased exponential phase in the culture having ABRF2 extract as compared with the positive controls, acarbose, and rapamycin as well as and negative control, nystatin (Fig. 7b).

Antiproliferative activity
Prediction of anticancer potential by molecular docking The docking interaction of the isolated compound, 3-methyl-4-oxo-pentanoic acid against, c-MYC was performed as described in the methods to identify and predict the binding site residues. The docked ligand molecules were selected based on the interaction with the active site residues and lowest docking energy. The presence of a total of five hydrogen bonds with binding sites of 3-methyl-4-oxo-pentanoic acid, including two hydrogen bonds formed by the Lys 62 were observed. The other three hydrogen bonds were observed to be formed by the residues Asn 108, Thr 102 and Ile 103, respectively (Fig. 8). The compound also interacted with Asn 61, Glu 44, Arg 84 and Asn 41 through the water of hydration. The binding energy was observed to be − 13.5751 kCal/mol for site 6 of 6AU4 from the c-MYC of DU-145. Compound, 3-methyl-4-oxo-pentanoic acid was most potent with a higher docking score of − 11.4592 kCal/ mol, compared to that of the standard drug, doxorubicin (− 10.088 kCal/mol). These findings confirm the plausible role of 3-methyl-4-oxo-pentanoic acid as an anticancer agent that can be evaluated in the future with the mechanism of action for potential drug development. The in vitro cytotoxic assay further confirmed the observed in silico activity of 3-methyl-4-oxo-pentanoic acid, with IC 50 value at a low micromolar range of the 3-methyl-4-oxopentanoic acid fraction against DU-145 of 2.36 ± 0.156, thereby suggesting potential antiproliferative/anticancer activity of the isolated compound (3-methyl-4-oxo-pentanoic acid) ( Table 4). The docking of compound against the anticancer site-5 on 1MP8, a well-known target of MCF-7, depicted a total of five hydrogen bonding with the residues Glu 506, Glu 500, Cys 502, Lys 454, Gln 432. The isolated compound also interacted with Glu 430, Ile 428, Leu 553, Lys 454, Phe 433 and Asp 564 through the water of hydration. The binding energy was observed to be − 13.2413 kCal/ mol for site 5 of 1MP8: transferase from the MCF-7 which was lower than that of the potent anticancer drug, doxorubicin with docking score of − 21.29661 kcal/mol (Fig. 8a).
1ELK of the VHS domain of TOM1 protein reported as target in MCF-7 cancer cell line is involved in the degradation of growth factor receptor complexes through their translocation to the lysosome. The binding energy of the compound, 3-methyl, 4-oxo-pentanoic acid was − 15.2661 kCal/mol for site 4 of the target 1ELK on MCF-7 breast cancer cell line as compared to the maximum affinity and lowest binding energy score of − 29.5353 kCal/mol for doxorubicin. Direct binding via Fig. 7 In vitro spot assay of anti-aging activity of the crude extract of secondary metabolites of Talaromyces purpureogenus isolate-ABRF2. S. cerevisiae BY4742 cells spotted onto YPD plates. Growth curve for the S. cerevisiae BY4742 strain were measured in YPD medium. p < 0.05 indicated a significant difference. Fungal extract and their growth spots generated during spot assay along with control systems using S. cerevisiae BY4742. C-culture only (S. cerevisiae BY4742); C + E2-culture and extract of isolate ABRF2; C+N-culture and fluconazole (nystatin) as negative control; C+VA-culture and n-valeric acid; C+Ac-culture and acarbose and C+Ra-culture and rapamycin as positive control hydrogen bonds with Val A59, Arg A57, Val A54, Leu A51, and Ala A53 was observed. Compound has displayed two hydrogen bonds with Val A59 and Asp A93 (Fig. 8b).
Similarly, site-1 of the 3SSU target from the MDA-MB-231 cell line displayed the highest docking with compound by three hydrogen bonding with Glu A187, Asp A181, and Arg A184. The binding energy of the isolated compound was observed to be − 7.7187 kCal/   (Fig. 8c). These binding affinities of the molecule with different amino acids of the target sites propose that the compound 3-methyl-4-oxo-pentanoic acid may act as a therapeutic potential drug on different anticancer pathway.

In vitro antiproliferative activity
To determine the antiproliferative activity of the crude fungal extract were further purified and segregated as extracellular and intracellular extracts to localize the selected metabolites. All three types of extracts were tested against tissue-specific cancer cell lines, using SRB assay as described in the methods. Intracellular extracts demonstrated lower IC 50 values and thus higher cytotoxicity (Table 3). The extract was further purified by adsorption column chromatography. The fractions were assessed for antiproliferative activity.
Fraction A and C (toluene and ethyl acetate fraction) depicted more antiproliferative potential against breast cancer, MCF-7 and liver cancer, HepG2 cell line with IC 50 of 2.79 and 2.75 µg/mL as compared with the positive control (doxorubicin) with IC 50 of 5.06 and 1.65 µg/ mL, respectively. MDA-MB-468 was observed to be highly sensitive with fraction B (IC 50 < 0.35 µg/mL) suggesting these fractions might contain efficacious anticancer lead molecules which can be further isolated, identified and purified as they did not show any comparable toxicity in normal primary cells (HEK-293) (Table 4). However, toluene and ethyl acetate fraction was also observed to be highly potent against liver cancer, but toluene fraction had similar toxicity in cancer as well as non-cancerous (control) cells with IC 50 of 2.75 and 2.29 µg/mL, respectively. Similarly, the antiproliferative potential of fraction C (ethyl acetate fraction) containing 3-methyl 4-oxo-pentanoic acid was observed to be comparable with cancer and normal cells.

Discussion
Identified fungus Talaromyces purpureogenus isolate-ABRF2 from the Achanakmar Biosphere Reserve forest of central India was characterized based on the morphological parameters such as variation in shape and size of fungal spores and hyphae (Wyatt et al. 2013;Gautier et al. 2016). The analysis of phenotypic characteristics and spore structure forms the major identifying principle in fungi. The preliminary identification was corroborated by sequence analysis of the ITS region of the strain. Talaromyces purpureogenus isolate-ABRF2 fungus was grown on Yeast Extract Sucrose Broth media (YESB) under optimized incubation condition. Both the intracellular and extracellular secondary metabolites were extracted and screened for therapeutic potential, the intracellular secondary metabolites depicted higher potential compared to extracellular and hence, the isolated intracellular compound was further evaluated. Successive Soxhlet extraction of 50 g dry biomass resulted in 0.45, 0.67, 1.2, 4.8 and 2.7 g dry extract, respectively, for solvents diethyl ether toluene, chloroform, ethanol, and acetonitrile. The ethanolic extract was selected and subjected to column chromatography, obtaining 1.7 g of purified active component. The isolated secondary metabolites showed various characteristic features. The R f value of Spots B, C, and D ranged from 0.30 to 0.40 which corresponds to valeric acid, or 3-methyl-4-oxo-pentanoic acid with R f = 0.49 (Hassan et al. 2008;Singh et al. 2006). 3-methyl-4-oxopentanoic acid is a straight-chain alkyl carboxylic acid sesquiterpenoid constituent of the essential oil of the valerian plant. Further, the presence of 3-methyl-4-oxopentanoic acid was confirmed using various spectral analysis. UV absorbance at λ max 272 and 328 nm having the typical pattern of 3-methyl-4-oxo-pentanoic acid. The IR spectrum showed absorption bands at 1716.32, 1652.84 and 1181.53 cm −1 revealed the similarity with 3-methyl-4-oxo-pentanoic acid reported earlier in the literature suggesting biotransformation of pyrone to benzoic acid and derivatives by microorganisms (Parshikov et al. 2015). The GC-MS analysis of a crude extract of ABRF2 revealed the presence of ester compound, which when further validated by 1 H NMR, showed chemical shift similar to standard n-valeric acid/pentanoic acid.
Antioxidants are molecules useful in reducing the free radicals produced by oxidative stress and managing the cellular network. Clinical data suggest a correlation between the pathogenesis of disease with a high level of iron in the body (Siah et al. 2006). In the Fenton reaction, ferrous ions play an important role, catalyzing the production of hydroxyl radicals and hydroxyl anions from hydrogen peroxide (Liochev and Fridovich 1999). In our present study, FRAP values of YESB crude extract were relatively higher and comparable to standard ascorbic acid. FRAP values are based on reducing ferric ion with antioxidants as the reducing agent, higher FRAP value indicating greater antioxidant capacity (Fernandes et al. 2016). DPPH scavenging activity of YSEB extract from isolate ABRF2 was similar to the reported T. purpureogenus CFRM02 extracts (Pandit et al. 2018). The variation in the structure and different functional groups present in the molecules led to differences in the antioxidant activity across different methods. The metabolic processes, irradiation and oxidative processes may lead to the formation of primary ROS which then react with biomolecules forming secondary ROS (Loganayaki et al. 2013). These free radicals damage macromolecules and cellular components, however, antioxidants protect biomolecules. ABTS + scavenging capacity represents freeradical scavenging efficiency in a hydrophilic medium (Re et al. 1999). Our data showed that ABTS + activity was slightly lower than FRAP but higher than DPPH activity. Phosphomolybdate assay is a routine method for estimating the reducing capacity of plant-derived antioxidants (Prieto et al. 1999). Interestingly, we observed the lowest activity of our compound in phosphomolybdate assay as compared with other antioxidant assays that were comparable with control, l-ascorbic acid.
The secondary metabolites of Talaromyces sp. predominantly containing esters including, linear polyesters are known to show various biological activities, including antibacterial actions (Zhai et al. 2016). The results obtained in the present study are comparable to the standard antibiotic streptomycin (Sarker et al. 2007). We observed a differential growth inhibition pattern of gram-positive by the ethyl acetate fraction containing the compound, 3-methyl, 4-oxo-pentanoic acid. Similarly, the zone of inhibition against gram-negative bacteria, R. eutropha was observed to be similar to that reported earlier of Alternaria sp (Palanichamy et al. 2018). Interestingly, antifungal activity against candida albicans was not observed in the ethyl acetate fraction.
The outcome of the initial pharmacokinetic studies of the isolated compound, 3-methyl-4-oxo-pentanoic acid incited us to further explore the anti-aging and anticancer activity. Literature suggests that 1AH8, 2KM1 and 2L7E are the targets associated with transcription and translational modification in eukaryotic system and are linked to the process of aging. Interestingly, the same signaling molecules have been shown to be involved and targeted for cancer therapy (Blagosklonny 2012). Rapamycin slows down aging, suppresses cellular senescence, and postpone age-related diseases (Blagosklonny 2012). Further to validate the anti-aging activity, we employed specific spot assay with eukaryotic model organism Saccharomyces cerevisiae mutant strain BY4742 (MTCC-3157) as reported earlier . Using the method of Wei et al. (2017), we observed that in the presence of ABRF2 crude extract, the formation of growth zone by yeast was higher in size as compared with control suggesting it's potential to enhance the lifespan and delay aging of yeast cell. In yeast growth curve determination, ABRF2 crude extract significantly enhanced the yeast growth exponential phase and delayed the aging process similar to the positive control. Anti-aging results suggested that fungal extracts have putative compounds responsible for the enhancement of cell life.
The c-MYC oncogene is often dysregulated or overexpressed in multiple tumor cell survival pathways (Stump et al. 2018). One of the major targets of anticancer compounds is the DNA quadruplex formed in the NHE III1 region of the c-MYC promoter. Antitumor small molecules tend to stabilize this quadruplex thereby reducing c-MYC expression (Stump et al. 2018). These observations were further validated using in vitro antiproliferative activity against a variety of tissue-specific cancer cell lines that demonstrated potent antiproliferative activity