Table 1 The formula of feed media used in the fed-batch cultures
Asn (mM) | Gln (mM) | Asn/Gln | Asn + Gln (mM) | ||
|---|---|---|---|---|---|
Total | T1 | 0 | 0 | / | 0 |
T2 | 30 | 30 | 1:1 | 60 | |
T3 | 60 | 60 | 1:1 | 120 | |
T4 | 90 | 90 | 1:1 | 180 | |
T5 | 120 | 120 | 1:1 | 240 | |
Proportion | P1 | 180 | 0 | / | 180 |
P2 | 150 | 30 | 5:1 | 180 | |
P3 | 120 | 60 | 2:1 | 180 | |
P4 | 90 | 90 | 1:1 | 180 | |
P5 | 60 | 120 | 1:2 | 180 | |
P6 | 30 | 150 | 1:5 | 180 | |
P7 | 0 | 180 | / | 180 | |
FB-L | 60 | 120 | 1:2 | 180 | |
FB-H | 120 | 60 | 2:1 | 180 | |
FB-N | 0 | 0 | / | 0 |