Fig. 3
From: Nitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture
Effects of red light on NO generation of Shiraia sp. S9 in liquid culture. A Bright-field image (left) and fluorescence microscopy of DAF-2 DA-stained mycelia (right) (400 ×) in the cultures. cPTIO (100 μM) were added 30 min before red light treatment and the photo was taken on day 8. The intensity of red light (627 nm) was 200 lx. B The NO content in the mycelium. l-NAME (100 μM), STD (100 μM) and cPTIO (100 μM) were added 30 min before red light treatment respectively, and the mycelia were harvested on day 8. C Time course of NO content. D NOS and NR activity in Shiraia sp. S9. E cGMP content in Shiraia sp. S9. NS-2028 (20 μM) and cPTIO (100 μM) were added 30 min prior to the red light treatment. The culture was maintained in a 150 mL flask containing 50 mL medium at 28 ℃ and 150 r/min. Values are mean ± SD from three independent experiments. (*p < 0.05 and **p < 0.01 vs. control. ##p < 0.01 vs. red light treatment, ten flasks per replicate)