Fig. 5
From: Nitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture
Effects of NO on hyphal cell membrane permeability of Shiraia sp. S9 under the red light. A The integrity of mycelial cell membrane. The mycelia cultured for 5 days were treated with 0.5 μM SYTOX Green for 30 min and photography through a fluorescent microscope. SNP (5 μM), cPTIO (100 μM) and NS-2028 (20 μM) were added 30 min prior to the red light treatment, respectively. The intensity of red light (627 nm) was 200 lx. B The mean fluorescence intensity. The culture was maintained in a 150 mL flask containing 50 mL medium at 28 ℃ and 150 r/min under the dark or red light treatment. Values are mean ± SD from three independent experiments (*p < 0.05 and **p < 0.01 vs. control. #p < 0.05 vs. red light treatment, ten flasks per replicate)